FastQCFastQC Report
Mon 6 Feb 2017
SRR2077377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674371
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.043.2548141
GTATCAA41350.040.009841
TATCAAC61050.027.0886362
TCAACGC61500.026.832444
ATCAACG61650.026.8233873
CAACGCA62350.026.4102445
AACGCAG64800.025.462156
ACCGTGC3100.023.3592038
ACGCAGA76150.021.5194787
ATACGTA1959.367795E-1021.22705725
CGTAGTA1304.948117E-621.21881562
CGCAGAG77850.020.9952078
GTACATG58400.020.6676161
GAGTACT52150.020.1684212
TACATGG59200.020.028982
AGAGTAC78500.019.68043511
AGTACTT54150.019.48719413
CAGAGTA81450.019.26401110
GCAGAGT82700.019.2635759
ATTATAC7150.018.424933
Produced by FastQC (version 0.11.5)