FastQCFastQC Report
Mon 6 Feb 2017
SRR2077376_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077376_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441518
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52730.21597219434794254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24580.1006750718200726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.024.3062171
GTATCAA38300.023.1344471
TAGGTCG1002.7988517E-617.9999645
TATCAAC49850.017.5071472
ATCAACG51100.017.1099933
TCAACGC50850.016.9952934
CAACGCA51350.016.7477745
CGCACGA1208.570223E-716.6707335
AACGCAG52350.016.3075646
TAGCCCG800.003755583214.9999695
ACGCAGA60950.013.7113577
CGCAGAG61700.013.6094968
GTATAAT6250.013.4711571
GTATTAG4200.013.36424351
TTAGACG1651.5844271E-613.3365854
CGATGCG900.00819426713.33494533
GTATAGT3800.013.1883981
AGAGTAC59900.013.15092211
GTATAGG4000.013.0301371
ATTATAC6250.012.8046953
Produced by FastQC (version 0.11.5)