FastQCFastQC Report
Mon 6 Feb 2017
SRR2077376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441518
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48900.039.5600621
GGTATCA22250.037.8623771
ATCAACG67400.028.5325873
TCAACGC67750.028.385194
CAACGCA68000.028.2785025
TATCAAC68200.028.2007982
AACGCAG71150.027.1715096
ACGCAGA83150.023.1968577
CGCAGAG85850.022.417478
AGAGTAC84150.021.60065811
GAGTACT56550.021.10347612
GTACATG64300.020.5421371
CAGAGTA92100.020.10978110
AGTACTT59050.020.09319713
GCAGAGT94700.019.921879
TACATGG65400.019.5524582
TATACCG1258.464145E-519.4026185
GTACTTT62100.018.93544214
ACATGGG67100.018.848573
CGAATCG1552.2573175E-517.79762537
Produced by FastQC (version 0.11.5)