FastQCFastQC Report
Mon 6 Feb 2017
SRR2077375_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077375_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637356
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66850.25347355457511234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.11731446190806248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30840.1169352942871573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.024.1578941
GTATCAA37150.023.2040961
ATCAACG50350.016.688063
TCAACGC50300.016.5837574
TATCAAC51000.016.4458122
CAACGCA51300.016.1778955
ACTACGC904.5725878E-415.5600713
AACGCAG53050.015.5270096
GTATTAC3250.014.8041291
CGTATAC1202.6474724E-413.3372043
ACGCAGA61300.013.2416337
GTACATG66000.013.0610921
TACATGG66650.012.8244642
ATTATAC5800.012.7623243
CGCAGAG63150.012.7270788
AGAGTAC62850.012.24704911
CTATACT5400.012.2246114
ACATGGG68500.012.0618953
GTATAAG5000.012.0283561
GAGTACT33400.011.91194412
Produced by FastQC (version 0.11.5)