FastQCFastQC Report
Mon 6 Feb 2017
SRR2077375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637356
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.040.7674451
GTATCAA54000.038.4555441
TATCAAC75150.027.8008252
ATCAACG76350.027.134913
TCAACGC76700.026.9202374
CAACGCA78200.026.4023555
AACGCAG82350.025.2807066
ACGCAGA93650.021.7360557
CGCAGAG96850.020.9030788
GTACATG69300.020.3104111
GAGTACT66400.020.05006212
AGAGTAC97700.019.91044811
TATGCGA1409.3737235E-619.70872112
TACATGG71950.019.2618732
CAGAGTA102500.019.21287210
AGTACTT71800.018.68622813
GCAGAGT106750.018.673769
ACATGGG74100.018.374293
GTACTTT75300.017.86077314
TACCGTA1552.2470502E-517.8071677
Produced by FastQC (version 0.11.5)