FastQCFastQC Report
Mon 6 Feb 2017
SRR2077374_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077374_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390849
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59060.2470252199114206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28520.11928816918174256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26810.11213589816839123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15400.023.4344371
GTATCAA34150.022.9556081
CGCACGA1052.11101E-719.05356835
TATCAAC45350.016.9487512
ATCAACG46300.016.2483333
TCAACGC46750.016.0895754
CAACGCA47450.015.8070785
AACGCAG48250.015.5802536
TACGCTC1201.5783022E-515.00468434
TACCGCG800.003763911514.99495729
CGCTATT956.925587E-414.73188811
GTATTAG3400.014.1525621
GTATAGG3750.013.9009611
GTGTACG2056.704795E-913.6923171
GTATAAA7700.013.5398971
CGTGCCG1651.5947553E-613.32885110
GTATTAA6700.013.1668121
ACGCAGA56400.013.04525857
GTACATG59300.013.0169371
GAGTACT30100.012.88603212
Produced by FastQC (version 0.11.5)