FastQCFastQC Report
Mon 6 Feb 2017
SRR2077374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390849
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.042.118761
GTATCAA49350.037.643391
TATCAAC70350.026.2466222
TCAACGC70650.026.0354614
ATCAACG70500.025.9925863
CAACGCA71250.025.5731245
AACGCAG73750.024.799666
ACGCAGA85650.021.3506097
CGCAGAG88500.020.6158
AGAGTAC89700.019.34076911
GAGTACT63200.019.10069512
CAGAGTA95050.018.43281410
GCAGAGT98150.018.1317399
AGTACTT67600.018.01051913
GTACATG58000.017.6280861
GTACTTT69000.017.39115514
CGTTCTA1602.9648862E-517.24159255
TACATGG58400.017.0246962
TATACTG8150.016.576395
GTGGTAT10400.015.9962811
Produced by FastQC (version 0.11.5)