FastQCFastQC Report
Mon 6 Feb 2017
SRR2077373_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077373_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640501
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68590.2597613104482823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31180.11808365154945975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.11441010626392491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA250.003760183231.98992519
GGTATCA15150.025.6778681
GTATCAA44000.025.4758681
TATCAAC56800.019.2837142
TTAGTCG951.7085131E-618.95396639
TAGTCGG951.7091334E-618.95324740
ATCAACG58950.018.499493
TCAACGC59400.018.356214
CAACGCA60650.018.0394175
AACGCAG62650.017.5194246
GTATAGA4950.017.0142961
ACGCAGA70750.015.2876027
CGCAGAG72200.014.92528
CGTATAC1505.39334E-714.6739993
GAGTACT35700.014.67241712
GGACCGT1557.889412E-714.1882746
GACCGTG2850.014.0298727
AGAGTAC73450.013.7729611
AGTACTT39150.013.63477813
CAGAGTA76350.013.51167710
Produced by FastQC (version 0.11.5)