FastQCFastQC Report
Mon 6 Feb 2017
SRR2077373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640501
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26900.043.4472471
GTATCAA61600.039.07181
TATCAAC86300.027.7568342
TCAACGC86050.027.7134534
ATCAACG87400.027.3255653
CAACGCA88000.027.058935
AACGCAG90900.026.4615696
ACGCAGA105200.022.531057
CGCAGAG107700.021.9036398
AGAGTAC108000.021.10911411
CGAATCG1154.623998E-520.989137
GAGTACT77650.020.60956612
CAGAGTA114150.020.18295110
GTACATG69400.019.9887281
GCAGAGT118150.019.7328079
AGTACTT83100.019.46543713
GTACTTT85700.018.9503514
TACATGG72250.018.6164382
TATACTG10450.017.579085
ACATGGG78200.016.9323793
Produced by FastQC (version 0.11.5)