FastQCFastQC Report
Mon 6 Feb 2017
SRR2077372_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077372_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534887
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50010.19728690075731187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.024.1889081
GTATCAA35900.022.7778071
GCGATAC751.14166454E-418.66070214
ATCAACG45850.017.3677463
TATCAAC46450.017.238062
TCAACGC46150.017.121744
CAACGCA47300.016.7024965
AACGCAG49050.016.1002336
ACGCAGA57050.013.6673087
CGTTAGA900.00815312913.3451312
TATACCG1508.720319E-613.3338075
CGCAGAG58400.013.1459658
ATCGTTA1100.002089677412.7267221
CGGCTTA1601.7007314E-512.49600514
AGAGTAC57650.012.10319911
GGACCGT1501.2916725E-411.9956926
GCAGAGT62700.011.8298839
TTGCGAG1200.00402013111.66247811
CAGAGTA61050.011.65838410
GTACATG59600.011.6016291
Produced by FastQC (version 0.11.5)