##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077372_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2534887 Sequences flagged as poor quality 0 Sequence length 46 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.357165822381827 31.0 31.0 34.0 26.0 34.0 2 30.484366758754927 31.0 31.0 34.0 26.0 34.0 3 30.571017958591447 31.0 31.0 34.0 26.0 34.0 4 34.00605904720802 37.0 35.0 37.0 30.0 37.0 5 34.05102199821925 37.0 35.0 37.0 30.0 37.0 6 34.1771562203759 37.0 35.0 37.0 30.0 37.0 7 34.1247716367633 37.0 35.0 37.0 30.0 37.0 8 34.08943238889939 37.0 35.0 37.0 30.0 37.0 9 35.61570910261483 39.0 37.0 39.0 30.0 39.0 10 35.55084782871978 39.0 37.0 39.0 30.0 39.0 11 35.57799420644786 39.0 37.0 39.0 30.0 39.0 12 35.503818118914175 39.0 37.0 39.0 30.0 39.0 13 35.46867848547095 39.0 37.0 39.0 30.0 39.0 14 36.55413949418653 40.0 37.0 41.0 28.0 41.0 15 36.693423809424246 40.0 37.0 41.0 29.0 41.0 16 36.66680605486556 40.0 37.0 41.0 29.0 41.0 17 36.67973483630631 40.0 37.0 41.0 29.0 41.0 18 36.641333124514034 40.0 37.0 41.0 29.0 41.0 19 36.739266484068125 40.0 38.0 41.0 29.0 41.0 20 36.73923571346573 40.0 38.0 41.0 30.0 41.0 21 36.752239448937964 40.0 38.0 41.0 30.0 41.0 22 36.70641176510038 40.0 37.0 41.0 29.0 41.0 23 36.66189617130862 40.0 37.0 41.0 29.0 41.0 24 36.689869410352415 40.0 37.0 41.0 30.0 41.0 25 36.636572754525154 40.0 37.0 41.0 29.0 41.0 26 36.66562612061208 40.0 37.0 41.0 30.0 41.0 27 36.646969273186535 40.0 37.0 41.0 30.0 41.0 28 36.5823127421459 40.0 37.0 41.0 29.0 41.0 29 36.55226130395556 40.0 37.0 41.0 29.0 41.0 30 36.46685355205183 40.0 37.0 41.0 29.0 41.0 31 36.43963932120051 40.0 37.0 41.0 29.0 41.0 32 36.41528872884669 40.0 37.0 41.0 29.0 41.0 33 36.37167810636135 40.0 37.0 41.0 29.0 41.0 34 36.31734432343532 40.0 37.0 41.0 29.0 41.0 35 36.293183483129624 40.0 37.0 41.0 29.0 41.0 36 36.234597439649185 40.0 37.0 41.0 29.0 41.0 37 36.15074123619711 40.0 37.0 41.0 28.0 41.0 38 36.11626317070544 40.0 36.0 41.0 28.0 41.0 39 36.041913899909545 40.0 36.0 41.0 28.0 41.0 40 36.01811323344985 40.0 36.0 41.0 28.0 41.0 41 35.88827470415841 40.0 36.0 41.0 27.0 41.0 42 35.83675919281609 40.0 36.0 41.0 27.0 41.0 43 35.736934624699245 40.0 36.0 41.0 27.0 41.0 44 35.6569271135163 40.0 36.0 41.0 27.0 41.0 45 35.55947385425859 40.0 36.0 41.0 27.0 41.0 46 35.455959575318346 40.0 35.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25474190975802813 1101 2 0.301427845813528 1101 3 0.45516350297570796 1101 4 0.4082316815625191 1101 5 0.3467747382190467 1101 6 0.31166164783074635 1101 7 0.4711524199983117 1101 8 0.5195125662422768 1101 9 0.4990054260825545 1101 10 0.5428756518489095 1101 11 0.468141490803724 1101 12 0.5374573547200328 1101 13 0.5178041163294651 1101 14 0.6922736879319231 1101 15 0.5069854910764136 1101 16 0.5737031260347933 1101 17 0.49054888800424123 1101 18 0.4915579575621578 1101 19 0.5526624901529331 1101 20 0.4794943671834915 1101 21 0.5351117045297258 1101 22 0.6131035989209579 1101 23 0.6223459736197015 1101 24 0.5469438568018248 1101 25 0.5629422996354876 1101 26 0.5996380564417692 1101 27 0.6225245899755336 1101 28 0.6767625396158508 1101 29 0.5169605968266353 1101 30 0.6655783374701869 1101 31 0.5975092786780678 1101 32 0.6242325477710651 1101 33 0.5839904761552575 1101 34 0.5914137119558447 1101 35 0.5330837963079773 1101 36 0.4484024215216067 1101 37 0.5729856607704775 1101 38 0.46805056061504047 1101 39 0.45600074456891804 1101 40 0.5059485381577531 1101 41 0.5091468605973546 1101 42 0.3450688682821266 1101 43 0.39294754438633106 1101 44 0.3874721881508023 1101 45 0.41501969214194645 1101 46 0.3901122174919749 1102 1 0.5535547170639035 1102 2 0.552952738633401 1102 3 0.6579474955134579 1102 4 0.7184351799861304 1102 5 0.6398173548058423 1102 6 0.6261628666934556 1102 7 0.6456376739553633 1102 8 0.6856596982190339 1102 9 0.7123611428610417 1102 10 0.808168033539431 1102 11 0.6747769163170148 1102 12 0.7377052715812837 1102 13 0.8875098263966308 1102 14 1.121832175713294 1102 15 0.9778738165303835 1102 16 0.9590757528710512 1102 17 0.9939880540435908 1102 18 0.945366122612441 1102 19 0.9433228347746905 1102 20 0.9958877768506369 1102 21 0.8862098871531572 1102 22 0.919829116976274 1102 23 0.9739655981444244 1102 24 0.9685760256228164 1102 25 0.9894581151601045 1102 26 0.9582747240135845 1102 27 0.8466878551049248 1102 28 0.8865900184287909 1102 29 0.9643082637297979 1102 30 0.8352512331923236 1102 31 0.8778402798527054 1102 32 0.9442025664081299 1102 33 0.9675600150410304 1102 34 0.9590209740394755 1102 35 0.9734281830137448 1102 36 0.9380747172453496 1102 37 0.9975234919061862 1102 38 0.879832085736723 1102 39 0.9989515057318314 1102 40 0.8562956403201625 1102 41 0.9284583655355902 1102 42 0.8691563294909983 1102 43 0.7294506114915151 1102 44 0.7052502023540654 1102 45 0.6488778797385564 1102 46 0.6612916968047102 1103 1 0.5294258379876311 1103 2 0.6149210180073332 1103 3 0.5583351711011524 1103 4 0.677512967621702 1103 5 0.6721303817015212 1103 6 0.7571528405193888 1103 7 0.7602084507532894 1103 8 0.7790043747292899 1103 9 0.9010799217462946 1103 10 0.7610317265435 1103 11 0.7666643103633035 1103 12 0.7965139816561049 1103 13 0.7412840575941289 1103 14 1.010215152756544 1103 15 1.0491633115149384 1103 16 1.0683918267783952 1103 17 0.8972634810922528 1103 18 0.9169444369411153 1103 19 0.793057882362703 1103 20 0.8591917646614178 1103 21 0.8314627543525361 1103 22 0.8018898615597294 1103 23 0.7910720449563726 1103 24 0.8668149406851171 1103 25 0.8187915029061159 1103 26 0.8716807797908999 1103 27 0.9130654028631042 1103 28 0.9783754623699323 1103 29 0.880327306595099 1103 30 0.9543830985816442 1103 31 0.8987762937862769 1103 32 0.8793648777525505 1103 33 0.9775009605899498 1103 34 1.0084804764227826 1103 35 1.0054172384047746 1103 36 1.0797651796362189 1103 37 1.0181676515647666 1103 38 1.0233849791308032 1103 39 1.0991978507763633 1103 40 0.9998592149029975 1103 41 0.9882333180448128 1103 42 0.9727993662251535 1103 43 0.9744792179249231 1103 44 0.945482218399718 1103 45 0.9739256798998497 1103 46 1.026434543340045 1104 1 0.4472882746608313 1104 2 0.4274595948972788 1104 3 0.5262871028878706 1104 4 0.47702835719749714 1104 5 0.4755021699834785 1104 6 0.47324466745619986 1104 7 0.579302781866815 1104 8 0.6137212967748766 1104 9 0.6539396546297453 1104 10 0.5963067073036683 1104 11 0.5818923566191572 1104 12 0.6324061199630719 1104 13 0.6711827383526341 1104 14 0.9904649481822645 1104 15 0.7902532902502628 1104 16 0.7554687440175698 1104 17 0.7663533161876117 1104 18 0.7048557380989493 1104 19 0.6825357968819503 1104 20 0.7795868734297997 1104 21 0.7274525169518711 1104 22 0.735025748093463 1104 23 0.6539489426703966 1104 24 0.7765975565709837 1104 25 0.6377960590408094 1104 26 0.736617268173184 1104 27 0.7638946438022103 1104 28 0.7334541274225401 1104 29 0.8011523902621107 1104 30 0.8501903308394034 1104 31 0.8673212492793283 1104 32 0.830871923416467 1104 33 0.8752468962367743 1104 34 0.9254522871521189 1104 35 0.888341506776861 1104 36 0.8652188271427264 1104 37 0.7246396715388599 1104 38 0.7649396069873475 1104 39 0.7716304699297467 1104 40 0.7820268074150221 1104 41 0.9431910667413135 1104 42 0.8967980499131514 1104 43 0.8728514703471575 1104 44 0.8324612627388888 1104 45 0.8313164177534915 1104 46 0.7544206463187066 1105 1 0.17294613397962877 1105 2 0.12330470785909853 1105 3 0.28801219887115437 1105 4 0.3186490224532932 1105 5 0.3503167040628412 1105 6 0.35209414208888035 1105 7 0.24203088269650408 1105 8 0.3475310415027053 1105 9 0.47498267357202906 1105 10 0.3404972529868928 1105 11 0.3437398513519696 1105 12 0.4817831625190152 1105 13 0.4660194843732981 1105 14 0.7493941049728505 1105 15 0.5931632776236029 1105 16 0.5876521225104625 1105 17 0.46591264494200146 1105 18 0.42287904691035294 1105 19 0.4600413947301334 1105 20 0.4857319984527564 1105 21 0.35332736952107524 1105 22 0.3803993220175528 1105 23 0.41084736693663615 1105 24 0.37221360799447467 1105 25 0.41430831096260334 1105 26 0.4448948625315765 1105 27 0.43772993068964894 1105 28 0.5350273803215941 1105 29 0.4880177103852432 1105 30 0.6221408700194786 1105 31 0.5288762667665523 1105 32 0.5622409856025357 1105 33 0.5643854585562238 1105 34 0.42762447173601004 1105 35 0.5780891315654912 1105 36 0.7217551956218742 1105 37 0.7073003729823597 1105 38 0.6568594378082508 1105 39 0.620071934193426 1105 40 0.4865329188088978 1105 41 0.5295871749758376 1105 42 0.5839339770847545 1105 43 0.6703249370936959 1105 44 0.5154707362734072 1105 45 0.613792891920248 1105 46 0.6051501521602063 1106 1 0.12755164769007976 1106 2 0.15835068781352746 1106 3 0.22577579340562437 1106 4 0.2960777977553306 1106 5 0.31517833438027054 1106 6 0.25525499888865966 1106 7 0.34888802832799115 1106 8 0.33224354023359837 1106 9 0.21695465592287633 1106 10 0.3432633757441437 1106 11 0.2138408217582679 1106 12 0.24612495424857883 1106 13 0.30370179879453474 1106 14 0.5391370744643922 1106 15 0.39353899109149637 1106 16 0.5600824992820534 1106 17 0.4841546897508735 1106 18 0.4394456532642792 1106 19 0.3689407674652827 1106 20 0.329642480088836 1106 21 0.3310307436596389 1106 22 0.3125828340067258 1106 23 0.4079208830283676 1106 24 0.3815231225799849 1106 25 0.417344677244607 1106 26 0.24275727551994208 1106 27 0.2753903971535365 1106 28 0.27302846072450393 1106 29 0.33530520080176984 1106 30 0.47384550334292186 1106 31 0.3416113554285616 1106 32 0.3460794375219649 1106 33 0.38105339373719005 1106 34 0.4355705595450914 1106 35 0.2282043759705914 1106 36 0.1745588954377979 1106 37 0.16432708303840116 1106 38 0.12454916482730738 1106 39 0.16250509408062896 1106 40 0.22584080693535924 1106 41 0.3082416635244414 1106 42 0.22435372632446615 1106 43 0.3545377030693686 1106 44 0.3277350942726116 1106 45 0.2810412902007968 1106 46 0.13347395830856357 1107 1 0.41823113296498704 1107 2 0.37277888425997574 1107 3 0.42541138699569103 1107 4 0.46869604000040965 1107 5 0.34410801170235317 1107 6 0.35029653685656115 1107 7 0.3012632280377261 1107 8 0.4117409923985207 1107 9 0.5306005535135441 1107 10 0.4426698105256932 1107 11 0.3768375142944649 1107 12 0.4667207505830362 1107 13 0.4317834587448459 1107 14 0.5866925665718128 1107 15 0.5518227271946543 1107 16 0.5411917394710812 1107 17 0.49399589945755196 1107 18 0.519868134961122 1107 19 0.5345336175116415 1107 20 0.5955539544865047 1107 21 0.3973229160321239 1107 22 0.42987713090973045 1107 23 0.5388216740252645 1107 24 0.5655629943902198 1107 25 0.4661101632054212 1107 26 0.38329218005965515 1107 27 0.45262840255791303 1107 28 0.5007584437757515 1107 29 0.6611438160077086 1107 30 0.39272869724071313 1107 31 0.5111475925630558 1107 32 0.5760114050514957 1107 33 0.4681072326649698 1107 34 0.4450071033475851 1107 35 0.4214699676885161 1107 36 0.5419225005236328 1107 37 0.5270752049523253 1107 38 0.41096661104995746 1107 39 0.5047335758227547 1107 40 0.534249242541982 1107 41 0.571182136355354 1107 42 0.549336067924898 1107 43 0.49933285182792275 1107 44 0.48002601965006875 1107 45 0.5539934819133023 1107 46 0.5674409322691218 1108 1 0.23285997087463173 1108 2 0.2606784530093833 1108 3 0.21873609460821442 1108 4 0.26972368289237636 1108 5 0.22893868828887065 1108 6 0.23706906399780792 1108 7 0.13584943535595784 1108 8 0.2686425969740043 1108 9 0.2699631097327071 1108 10 0.37472032708532055 1108 11 0.24628531574586532 1108 12 0.16137475319573724 1108 13 0.17046652012960095 1108 14 0.28386848927316777 1108 15 0.3948563632523303 1108 16 0.2507138121536343 1108 17 0.25010138385283653 1108 18 0.4148094873259822 1108 19 0.4053550299995692 1108 20 0.4093715723307412 1108 21 0.34774252270138106 1108 22 0.37690125722983936 1108 23 0.26772290748384364 1108 24 0.45080399972356133 1108 25 0.3692245942826915 1108 26 0.21955120476657441 1108 27 0.2972775668271197 1108 28 0.3488734038806882 1108 29 0.44700843596645967 1108 30 0.13515868348599014 1108 31 0.23011786014472335 1108 32 0.3599331110833148 1108 33 0.26987471676356733 1108 34 0.4218501799172998 1108 35 0.29736738736271207 1108 36 0.29590669030579875 1108 37 0.3310527752425543 1108 38 0.24991370775142485 1108 39 0.27936287098244605 1108 40 0.3211101093285649 1108 41 0.25084910413358585 1108 42 0.2984106682697458 1108 43 0.32958129810822356 1108 44 0.2643790560296253 1108 45 0.34443179861638384 1108 46 0.3484003492008796 1109 1 0.2802029707150808 1109 2 0.22544639842819691 1109 3 0.35544857184024536 1109 4 0.3560628758645237 1109 5 0.3656498588005803 1109 6 0.38849972020531 1109 7 0.3427734327127254 1109 8 0.3100703352750287 1109 9 0.3673583979867132 1109 10 0.4510578174008941 1109 11 0.4386819152937136 1109 12 0.4309048970774043 1109 13 0.46936905800259865 1109 14 0.6217936813643163 1109 15 0.4188418027291618 1109 16 0.5360153515877784 1109 17 0.38938561734441635 1109 18 0.4164377915153139 1109 19 0.46467425223440983 1109 20 0.19475667652927342 1109 21 0.4483110505777148 1109 22 0.3104502821747346 1109 23 0.24549337343548672 1109 24 0.3402740161764797 1109 25 0.40299684625966137 1109 26 0.40271739332605705 1109 27 0.342550917690545 1109 28 0.3051920303817326 1109 29 0.358326228126586 1109 30 0.44075267476781477 1109 31 0.45486767873926937 1109 32 0.5210347390346897 1109 33 0.38265036451463175 1109 34 0.47310425900811737 1109 35 0.4882729536907249 1109 36 0.42273454702182534 1109 37 0.45335924898328983 1109 38 0.43596559212706154 1109 39 0.5648689121464869 1109 40 0.4961930566676003 1109 41 0.5632382370815279 1109 42 0.5705166192439535 1109 43 0.4696865439761453 1109 44 0.6065469729880704 1109 45 0.4413687547185532 1109 46 0.49038505090342 1110 1 0.30673619245684236 1110 2 0.21647075379206981 1110 3 0.23810613524826252 1110 4 0.2110139980007446 1110 5 0.22247874794918232 1110 6 0.23307866935951438 1110 7 0.1963711928385905 1110 8 0.26824649162387715 1110 9 0.22200810977130914 1110 10 0.29453228642983476 1110 11 0.3073148808021102 1110 12 0.30162178497939607 1110 13 0.31339620692583026 1110 14 0.3001052053307447 1110 15 0.4577210362659514 1110 16 0.4099481629037669 1110 17 0.29197440364780647 1110 18 0.42712092547923675 1110 19 0.3267242573238889 1110 20 0.3347615649439817 1110 21 0.4014824881612 1110 22 0.3448886611311792 1110 23 0.36862154415048565 1110 24 0.3650296896907008 1110 25 0.34995660417631314 1110 26 0.40251236894053477 1110 27 0.3703384136535135 1110 28 0.40129430506539876 1110 29 0.5289821679274525 1110 30 0.32646392654127965 1110 31 0.3798712916421749 1110 32 0.4346787568843169 1110 33 0.44785239176157177 1110 34 0.3597330624523707 1110 35 0.3024105883263317 1110 36 0.37652287992160893 1110 37 0.3204861052299677 1110 38 0.4276974481286899 1110 39 0.4916857624742903 1110 40 0.42283969214392414 1110 41 0.4410841678218702 1110 42 0.3817311326833206 1110 43 0.2729189296607544 1110 44 0.27673926738876276 1110 45 0.37467067150491573 1110 46 0.15827325574935713 1111 1 -0.46534033343106884 1111 2 -0.2406388088050413 1111 3 -0.6392633265908074 1111 4 -0.526248428039402 1111 5 -0.6762908111951589 1111 6 -0.5975811231605306 1111 7 -0.42982040438035796 1111 8 -0.35359932660360016 1111 9 -0.6043074731971245 1111 10 -0.6380241741693808 1111 11 -0.8059751359908915 1111 12 -0.5394787062552808 1111 13 -0.62411026701362 1111 14 -0.7804227542404831 1111 15 -0.6347542842921428 1111 16 -0.6090844343622734 1111 17 -0.3376880098368247 1111 18 -0.3819739785495173 1111 19 -0.30413406933932663 1111 20 -0.25087021307444246 1111 21 -0.2825853430298437 1111 22 -0.29246793130098325 1111 23 -0.2721462258324081 1111 24 -0.2304809275699995 1111 25 -0.16042916414297537 1111 26 -0.28608408098648397 1111 27 -0.39738370132364764 1111 28 -0.4194521205353041 1111 29 -0.6283345607320854 1111 30 -0.43386455722286144 1111 31 -0.7037735755047407 1111 32 -0.8064358593091612 1111 33 -0.8009242298621686 1111 34 -0.6400872366828665 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-0.7921974335918733 2215 33 -0.7473106501716202 2215 34 -0.7681057435177792 2215 35 -0.7588815225478669 2215 36 -0.9150629054373098 2215 37 -0.7528512082028698 2215 38 -0.6571341083745068 2215 39 -0.8329564550097217 2215 40 -0.7493462353614078 2215 41 -0.5408018307566707 2215 42 -0.7356707152200173 2215 43 -0.692031176949051 2215 44 -0.7940414006993706 2215 45 -0.8968028181894709 2215 46 -1.0339510512868912 2216 1 -1.429784813118573 2216 2 -1.5168634890703991 2216 3 -1.3099349663734259 2216 4 -1.201498621716027 2216 5 -1.2500912175796657 2216 6 -1.4037033710781515 2216 7 -1.5495750357988456 2216 8 -1.5374248773084247 2216 9 -2.0618250098226767 2216 10 -1.9841401648421453 2216 11 -1.9400486216126893 2216 12 -2.2723777329701633 2216 13 -2.1511899118404827 2216 14 -4.0489823095627955 2216 15 -2.926796049238881 2216 16 -2.5314595153412043 2216 17 -2.479269468399636 2216 18 -2.6810366413484132 2216 19 -2.414139749879581 2216 20 -2.3933888166034905 2216 21 -2.428650439522137 2216 22 -2.2835818746724357 2216 23 -2.405517813903849 2216 24 -2.33015662735297 2216 25 -2.3995306487728314 2216 26 -2.252633292150719 2216 27 -2.4949371281984654 2216 28 -2.4376224002562523 2216 29 -2.650678259369336 2216 30 -2.415204559183657 2216 31 -2.4320469507837217 2216 32 -2.368672857984663 2216 33 -2.316016108515832 2216 34 -2.2399006246877917 2216 35 -2.2217606032477875 2216 36 -2.576933785562666 2216 37 -2.353266930323521 2216 38 -2.552583050657084 2216 39 -2.4319574474359413 2216 40 -2.4559946553143988 2216 41 -2.4611337189781324 2216 42 -2.4335992082991496 2216 43 -2.3300722842877803 2216 44 -2.388623589260348 2216 45 -2.392620159531859 2216 46 -2.6256887895997636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73346.0 3 2303.0 4 6940.0 5 5395.0 6 4347.0 7 3641.0 8 3228.0 9 2891.0 10 2691.0 11 2646.0 12 2773.0 13 2681.0 14 2858.0 15 3086.0 16 3281.0 17 3568.0 18 4082.0 19 4911.0 20 6903.0 21 9820.0 22 12721.0 23 14840.0 24 15821.0 25 16495.0 26 18072.0 27 21094.0 28 23747.0 29 26279.0 30 29615.0 31 35622.0 32 44466.0 33 56283.0 34 72898.0 35 98378.0 36 135885.0 37 199730.0 38 366877.0 39 1194673.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50659360291665 18.542245382264543 13.831111750541892 24.120049264276915 2 15.219004405585407 20.523034004551622 39.18093686380102 25.077024726061946 3 18.05674092589683 25.573010874001955 29.936625561839225 26.433622638261987 4 12.459002545601958 17.360342213532938 38.76056934012108 31.42008590074402 5 13.790136675545256 37.71082373547198 34.90864156797804 13.590398021004724 6 31.05751854027418 37.61942840055592 18.175445295983607 13.14760776318629 7 27.485761692730286 32.45406994473521 22.362259146068446 17.69790921646606 8 26.10538457927316 34.42962151764556 20.65472740993977 18.81026649314151 9 26.466860258465168 15.902089521150254 19.693856175837425 37.93719404454715 10 14.245684324390002 29.07238074123225 34.38109864463386 22.300836289743884 11 33.61704091740579 23.656320774851107 24.505944446438836 18.22069386130427 12 23.056767421979757 25.130942720523635 30.722276772100688 21.090013085395917 13 27.252575755842372 22.61229001529457 26.36224021031312 23.77289401854994 14 22.094633804189296 21.656941709827695 27.5703413998336 28.678083086149403 15 23.285808457431763 29.24213859180088 24.17034632367648 23.301706627090876 16 23.10083250259282 28.65145468022835 25.668520924206877 22.579191892971956 17 22.121007700561762 28.31179385217446 26.885572492583478 22.6816259546803 18 22.087317263876542 28.207786510635056 27.586768715252173 22.11812751023622 19 22.942120708720218 27.72948925767265 27.67323410703233 21.6551559265748 20 23.283017185706864 27.159002997823528 27.556819190489023 22.001160625980585 21 23.398420009270858 26.99689327665618 27.45716173700354 22.147524977069423 22 23.175707635093794 26.791963507643533 27.359562773409625 22.67276608385305 23 23.020391868184845 26.934852614499366 27.173995801778624 22.87075971553717 24 22.670992450129752 27.001220571562023 27.413705906161955 22.91408107214627 25 23.012671640748867 26.89838899226904 27.35336247614772 22.735576890834373 26 22.808194805737877 27.052705578265428 27.392012384047494 22.7470872319492 27 22.9081738750253 27.30330822801318 27.122979240069178 22.665538656892338 28 22.819389516248336 27.106070319049262 27.289905813896148 22.78463435080625 29 23.053253224031213 27.193071045063455 27.168414948301095 22.585260782604237 30 23.045342005974277 27.204020090986987 27.182717206576434 22.567920696462302 31 23.13345486550129 26.80241922917151 27.21790617774494 22.846219727582266 32 22.913968471004267 27.08376317077818 27.17982321007798 22.822445148139572 33 22.958217547115325 26.937634031916758 27.140447301687992 22.963701119279925 34 23.136241411632525 26.93334921872288 27.25132001275039 22.679089356894206 35 23.200654242993043 27.079324201388804 27.030760940146862 22.689260615471284 36 23.106082429720225 27.016210183869056 27.1265458236224 22.75116156278832 37 23.208655266446996 26.79603959146057 27.177822280296432 22.817482861796005 38 23.029180621898664 26.944010340121665 27.188309918002613 22.838499119977055 39 23.188692947695806 27.177262752403657 27.009875120380165 22.624169179520372 40 23.268686584925106 26.867672101549346 27.240251695408784 22.623389618116764 41 23.17319006449387 26.969048546647826 27.048151134889252 22.809610253969044 42 23.05488824908167 27.03309620818173 27.106002126784457 22.806013415952147 43 23.115065311519555 26.81065203400311 27.1684320223941 22.90585063208324 44 23.10734427606106 27.03033426408078 26.964276976549268 22.898044483308894 45 23.197024702075606 27.040091547628442 26.831228790150735 22.931654960145213 46 23.22526090168126 26.894102012083863 26.789378318486296 23.091258767748574 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 761.0 1 707.5 2 654.0 3 820.5 4 987.0 5 1024.0 6 1061.0 7 1061.0 8 1050.0 9 1039.0 10 1247.0 11 1455.0 12 1993.0 13 2531.0 14 4615.5 15 6700.0 16 9427.0 17 12154.0 18 13571.5 19 14989.0 20 14989.0 21 17563.0 22 20137.0 23 25069.5 24 30002.0 25 37261.5 26 44521.0 27 51709.5 28 58898.0 29 66844.5 30 74791.0 31 74791.0 32 83358.0 33 91925.0 34 101421.5 35 110918.0 36 120379.0 37 129840.0 38 137595.5 39 145351.0 40 154037.5 41 162724.0 42 168913.5 43 175103.0 44 175103.0 45 179460.5 46 183818.0 47 186517.0 48 189216.0 49 190500.0 50 191784.0 51 186870.5 52 181957.0 53 174951.5 54 167946.0 55 156563.0 56 145180.0 57 145180.0 58 132943.0 59 120706.0 60 106701.0 61 92696.0 62 79389.5 63 66083.0 64 55296.5 65 44510.0 66 36122.5 67 27735.0 68 22144.5 69 16554.0 70 16554.0 71 12864.5 72 9175.0 73 7170.0 74 5165.0 75 4021.0 76 2877.0 77 2132.5 78 1388.0 79 1069.0 80 750.0 81 750.0 82 578.0 83 406.0 84 294.5 85 183.0 86 146.0 87 109.0 88 78.5 89 48.0 90 32.5 91 17.0 92 15.5 93 14.0 94 14.0 95 9.5 96 5.0 97 7.5 98 10.0 99 12.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1916850731413274 2 0.05069259497563402 3 0.06663018903801235 4 0.05909533639961071 5 0.04244765151267098 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.5779796101364677E-4 16 0.0 17 2.7614643177388185E-4 18 6.311918440545871E-4 19 3.1559592202729355E-4 20 0.001893575532163761 21 0.0024458683957115247 22 0.0 23 1.5779796101364677E-4 24 3.550454122807052E-4 25 5.522928635477637E-4 26 5.12843373294352E-4 27 0.024853178859649362 28 4.7339388304094024E-4 29 6.706413343079987E-4 30 7.49540314814822E-4 31 0.0011834847076023507 32 5.522928635477637E-4 33 0.001696328080896703 34 0.0024853178859649363 35 0.0019330250224171728 36 0.03159904169298276 37 0.007850448560428927 38 0.011598150134503037 39 0.04919351434600437 40 0.015582548650097618 41 0.05791185169200837 42 0.05858249302631636 43 0.025563269684210778 44 0.02852198145321665 45 0.027101799804093833 46 0.0236302446617936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 2534887.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.06194115327071 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33239204713264 51.74789873812606 2 12.958529412136278 17.121312149149485 3 4.1349986576517574 8.194981139719314 4 1.7369944759328428 4.58996907410527 5 0.8641448176873665 2.8543542046983323 6 0.5203287585351402 2.0624356696021704 7 0.32871121959496885 1.520071087171187 8 0.22776606461889498 1.2037334686052397 9 0.15441953645050122 0.9181128896917516 >10 0.712234768031932 7.83263177161935 >50 0.023946735059540796 1.0253731863879014 >100 0.0052940543471387625 0.5230601257750089 >500 5.986320523955002E-5 0.02443991956820856 >1k 1.1972641047873156E-4 0.18385305189684775 >5k 5.986320523936578E-5 0.19777352388387512 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5001 0.19728690075731187 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.9449490253411694E-5 3 0.0 0.0 0.0 0.0 3.9449490253411694E-5 4 0.0 0.0 0.0 0.0 3.9449490253411694E-5 5 3.9449490253411694E-5 0.0 0.0 0.0 3.9449490253411694E-5 6 7.889898050682339E-5 0.0 0.0 0.0 7.889898050682339E-5 7 1.5779796101364677E-4 0.0 0.0 0.0 7.889898050682339E-5 8 1.5779796101364677E-4 0.0 0.0 3.9449490253411694E-5 7.889898050682339E-5 9 1.5779796101364677E-4 0.0 0.0 3.9449490253411694E-5 7.889898050682339E-5 10 1.5779796101364677E-4 0.0 0.0 1.5779796101364677E-4 7.889898050682339E-5 11 1.5779796101364677E-4 0.0 0.0 1.5779796101364677E-4 7.889898050682339E-5 12 1.9724745126705845E-4 0.0 0.0 1.9724745126705845E-4 7.889898050682339E-5 13 1.9724745126705845E-4 0.0 0.0 1.9724745126705845E-4 7.889898050682339E-5 14 3.1559592202729355E-4 0.0 0.0 2.3669694152047015E-4 7.889898050682339E-5 15 3.1559592202729355E-4 0.0 0.0 2.3669694152047015E-4 1.1834847076023507E-4 16 3.1559592202729355E-4 0.0 0.0 2.3669694152047015E-4 1.1834847076023507E-4 17 3.1559592202729355E-4 0.0 0.0 2.3669694152047015E-4 1.1834847076023507E-4 18 3.1559592202729355E-4 0.0 0.0 2.3669694152047015E-4 1.1834847076023507E-4 19 4.339443927875286E-4 0.0 0.0 2.3669694152047015E-4 1.1834847076023507E-4 20 4.339443927875286E-4 0.0 0.0 2.3669694152047015E-4 1.1834847076023507E-4 21 4.339443927875286E-4 0.0 0.0 3.1559592202729355E-4 1.1834847076023507E-4 22 4.339443927875286E-4 0.0 0.0 3.550454122807052E-4 1.1834847076023507E-4 23 4.339443927875286E-4 0.0 0.0 3.550454122807052E-4 1.5779796101364677E-4 24 4.733938830409403E-4 0.0 0.0 3.944949025341169E-4 1.5779796101364677E-4 25 4.733938830409403E-4 0.0 0.0 4.339443927875286E-4 1.5779796101364677E-4 26 4.733938830409403E-4 0.0 0.0 5.522928635477637E-4 1.5779796101364677E-4 27 4.733938830409403E-4 0.0 0.0 6.311918440545871E-4 1.5779796101364677E-4 28 5.522928635477637E-4 0.0 0.0 0.0011045857270955274 1.5779796101364677E-4 29 5.522928635477637E-4 0.0 0.0 0.001656878590643291 1.5779796101364677E-4 30 5.522928635477637E-4 0.0 0.0 0.0024853178859649363 1.5779796101364677E-4 31 5.522928635477637E-4 0.0 0.0 0.0046550398499025794 1.9724745126705845E-4 32 5.522928635477637E-4 0.0 0.0 0.0069036607943470454 1.9724745126705845E-4 33 5.522928635477637E-4 0.0 0.0 0.009152281738791512 1.9724745126705845E-4 34 5.917423538011753E-4 0.0 0.0 0.012505488410331505 1.9724745126705845E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1450 0.0 24.188908 1 GTATCAA 3590 0.0 22.777807 1 GCGATAC 75 1.14166454E-4 18.660702 14 ATCAACG 4585 0.0 17.367746 3 TATCAAC 4645 0.0 17.23806 2 TCAACGC 4615 0.0 17.12174 4 CAACGCA 4730 0.0 16.702496 5 AACGCAG 4905 0.0 16.100233 6 ACGCAGA 5705 0.0 13.667308 7 CGTTAGA 90 0.008153129 13.345131 2 TATACCG 150 8.720319E-6 13.333807 5 CGCAGAG 5840 0.0 13.145965 8 ATCGTTA 110 0.0020896774 12.72672 21 CGGCTTA 160 1.7007314E-5 12.496005 14 AGAGTAC 5765 0.0 12.103199 11 GGACCGT 150 1.2916725E-4 11.995692 6 GCAGAGT 6270 0.0 11.829883 9 TTGCGAG 120 0.004020131 11.662478 11 CAGAGTA 6105 0.0 11.658384 10 GTACATG 5960 0.0 11.601629 1 >>END_MODULE