FastQCFastQC Report
Mon 6 Feb 2017
SRR2077372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534887
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.043.7665561
GTATCAA52250.038.328891
TATCAAC70650.028.240082
TCAACGC70250.028.152674
ATCAACG71400.027.748293
CAACGCA71600.027.5729395
AACGCAG74500.026.4964796
ACGCAGA86850.022.7224677
CGCAGAG89800.021.8145898
AGAGTAC91950.020.33131411
GAGTACT63050.020.02229512
CAGAGTA98000.019.32063110
GCAGAGT100250.019.1966239
AGTACTT67100.018.865213
GTACTTT69400.017.8892614
GTACATG61700.017.2400261
TAGCGCG1250.001945791816.55450618
TACATGG61100.016.5537832
CTCGTAT3551.8189894E-1216.51336739
GTGGTAT11750.016.2184581
Produced by FastQC (version 0.11.5)