FastQCFastQC Report
Mon 6 Feb 2017
SRR2077371_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077371_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488049
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48080.193243782578237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35200.022.7762641
GGTATCA14750.022.2852231
TATCAAC44600.017.4148562
ATCAACG45150.017.0604363
TCAACGC45350.016.8063624
CAACGCA46650.016.3779355
AACGCAG47850.016.0038576
CGAATCG1151.05576E-515.65563637
GTATAGG4900.014.7255531
CCGCGAA850.00560915414.120769537
GTCCTAA3850.014.0562071
TAATTCG1000.001019305313.99859325
CGCACGA1151.8468549E-413.91612135
GTGGTAT6550.013.1580731
ACGCAGA56850.013.153737
TAATACG1406.741923E-512.8599874
CGCAGAG58850.012.6387548
GTATTAG4000.012.5269441
GAGTACT27150.012.44610212
AGAGTAC56900.012.29882311
Produced by FastQC (version 0.11.5)