##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077371_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2488049
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.264107338722027	31.0	31.0	34.0	26.0	34.0
2	30.298130784401753	31.0	31.0	34.0	26.0	34.0
3	30.427290218158888	33.0	31.0	34.0	26.0	34.0
4	33.847270692819954	37.0	35.0	37.0	28.0	37.0
5	33.87535575063031	37.0	35.0	37.0	30.0	37.0
6	33.93183574760786	37.0	35.0	37.0	30.0	37.0
7	33.80304608148794	37.0	35.0	37.0	29.0	37.0
8	33.74204286169605	37.0	35.0	37.0	28.0	37.0
9	35.25603595427582	39.0	37.0	39.0	28.0	39.0
10	35.19479600281184	39.0	37.0	39.0	28.0	39.0
11	35.23860583131602	39.0	37.0	39.0	28.0	39.0
12	35.16606184202964	39.0	37.0	39.0	27.0	39.0
13	35.13626741273986	39.0	37.0	39.0	27.0	39.0
14	36.19180369839983	40.0	37.0	41.0	27.0	41.0
15	36.33741698817025	40.0	37.0	41.0	27.0	41.0
16	36.30860284504043	40.0	37.0	41.0	27.0	41.0
17	36.32458645308031	40.0	37.0	41.0	27.0	41.0
18	36.288587162069554	40.0	37.0	41.0	27.0	41.0
19	36.38344944171116	40.0	37.0	41.0	27.0	41.0
20	36.37604524669731	40.0	37.0	41.0	27.0	41.0
21	36.392413091542814	40.0	37.0	41.0	27.0	41.0
22	36.33490859705737	40.0	37.0	41.0	27.0	41.0
23	36.28784240181765	40.0	37.0	41.0	27.0	41.0
24	36.31330371708917	40.0	37.0	41.0	27.0	41.0
25	36.25681889705548	40.0	37.0	41.0	27.0	41.0
26	36.28276935060362	40.0	37.0	41.0	27.0	41.0
27	36.26335775541398	40.0	37.0	41.0	27.0	41.0
28	36.19837350470188	40.0	37.0	41.0	27.0	41.0
29	36.16183041411162	40.0	37.0	41.0	27.0	41.0
30	36.076109433536075	40.0	37.0	41.0	27.0	41.0
31	36.052673399920984	40.0	37.0	41.0	27.0	41.0
32	36.02667793118222	40.0	36.0	41.0	27.0	41.0
33	35.98291995053152	40.0	36.0	41.0	27.0	41.0
34	35.931119925692784	40.0	36.0	41.0	27.0	41.0
35	35.900120536211304	40.0	36.0	41.0	27.0	41.0
36	35.83697628141568	40.0	36.0	41.0	27.0	41.0
37	35.75233687117898	40.0	36.0	41.0	26.0	41.0
38	35.71600117200264	40.0	36.0	41.0	26.0	41.0
39	35.63573506791868	40.0	36.0	41.0	26.0	41.0
40	35.61272185555831	40.0	36.0	41.0	26.0	41.0
41	35.48607804749826	40.0	36.0	41.0	25.0	41.0
42	35.43907495390967	40.0	35.0	41.0	25.0	41.0
43	35.32905059345696	40.0	35.0	41.0	25.0	41.0
44	35.247672373011945	40.0	35.0	41.0	24.0	41.0
45	35.1511184064301	40.0	35.0	41.0	24.0	41.0
46	35.05125662718057	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4022368998196306
1101	2	0.40194115130242736
1101	3	0.539229061981338
1101	4	0.46058614008719445
1101	5	0.4442677821013987
1101	6	0.39798876330844024
1101	7	0.5066508575113104
1101	8	0.6160727349344555
1101	9	0.6458120497433839
1101	10	0.5606409046236251
1101	11	0.6155740013569826
1101	12	0.6924316398444432
1101	13	0.6632027836606156
1101	14	0.8238208292436653
1101	15	0.7089162202229602
1101	16	0.7354582932222868
1101	17	0.68436335619419
1101	18	0.7721307820246679
1101	19	0.6342008704881508
1101	20	0.5661616494421153
1101	21	0.5078271415757527
1101	22	0.7319816873560754
1101	23	0.6804934477676383
1101	24	0.7251756265118985
1101	25	0.7080228211467272
1101	26	0.7279791832663349
1101	27	0.7006673517220037
1101	28	0.6136936245129405
1101	29	0.6485713330067497
1101	30	0.7064839444034021
1101	31	0.714183993868815
1101	32	0.6706431664679684
1101	33	0.744588281273117
1101	34	0.6227779259681938
1101	35	0.7209952666770718
1101	36	0.5656319916523813
1101	37	0.6202718206719453
1101	38	0.5420818053861964
1101	39	0.6539594585362849
1101	40	0.6344290301617974
1101	41	0.6835539522931029
1101	42	0.5291211741476403
1101	43	0.5403889788708085
1101	44	0.5605931310437029
1101	45	0.5423076565594656
1101	46	0.5306633545801844
1102	1	0.4900452223046834
1102	2	0.553698643326026
1102	3	0.5843251802159344
1102	4	0.5832177362594635
1102	5	0.500265391830311
1102	6	0.5666158902011844
1102	7	0.48545132850208006
1102	8	0.4931084503217349
1102	9	0.677542474631025
1102	10	0.7133839832089706
1102	11	0.6516646932147339
1102	12	0.7123343196463026
1102	13	0.6488769321677452
1102	14	0.9549554051318339
1102	15	0.9105308304488986
1102	16	0.8450109720705754
1102	17	0.7244974016173416
1102	18	0.7133986758007609
1102	19	0.7650223001443237
1102	20	0.6532405531100878
1102	21	0.6290128497700849
1102	22	0.7356316068209807
1102	23	0.8083814271197696
1102	24	0.7382135758325958
1102	25	0.7473290303656128
1102	26	0.7750954478697167
1102	27	0.7536622893482203
1102	28	0.72506702502438
1102	29	0.7827766255547886
1102	30	0.7381154810072559
1102	31	0.7214741794185571
1102	32	0.743336179327919
1102	33	0.818492204712868
1102	34	0.7800938813337908
1102	35	0.7912452680308988
1102	36	0.7120744738707359
1102	37	0.8046224201350753
1102	38	0.7870669332946605
1102	39	0.8363876226729445
1102	40	0.7918490536736655
1102	41	0.8772092329156891
1102	42	0.8403741664011548
1102	43	0.8170654472499805
1102	44	0.8489560755318237
1102	45	0.8182151508080509
1102	46	0.7677155230943029
1103	1	0.5741422586449723
1103	2	0.5459167506205276
1103	3	0.6461662671476063
1103	4	0.6427722917542127
1103	5	0.5521514581394982
1103	6	0.5471646622671145
1103	7	0.5932960414830433
1103	8	0.5360567368849658
1103	9	0.7164971488641569
1103	10	0.7239581644449729
1103	11	0.6804453763078087
1103	12	0.7075860652881545
1103	13	0.6122346348249152
1103	14	0.8729265325203031
1103	15	0.9039541798041526
1103	16	0.8875436215467758
1103	17	0.797477050093562
1103	18	0.7870108973998242
1103	19	0.7124252954892043
1103	20	0.7659956008288589
1103	21	0.8175343338597187
1103	22	0.793413450548222
1103	23	0.762310587821446
1103	24	0.7797494046009703
1103	25	0.8059411687016294
1103	26	0.8187959652233587
1103	27	0.8444537502214047
1103	28	0.8903459867575094
1103	29	0.9304270289510796
1103	30	0.9548219217921954
1103	31	0.9624985545444389
1103	32	1.0028065976995038
1103	33	1.0275848612660496
1103	34	1.110480456225737
1103	35	1.0348868155541737
1103	36	1.087434624235513
1103	37	1.039836616778885
1103	38	1.0031413284149835
1103	39	1.002970009049207
1103	40	0.9686985652435709
1103	41	1.0279980881452033
1103	42	1.0299279616025316
1103	43	1.0898771680952777
1103	44	1.0187719315743067
1103	45	1.1017995278870387
1103	46	0.9752222634453744
1104	1	0.5892877300614217
1104	2	0.5038050610556866
1104	3	0.5598090872536616
1104	4	0.6010719283019341
1104	5	0.5703266048504005
1104	6	0.5752760683283
1104	7	0.5878409425103968
1104	8	0.5886914325926611
1104	9	0.639388996391034
1104	10	0.7306998307149897
1104	11	0.8065685993912908
1104	12	0.7626911757953607
1104	13	0.8282419245783927
1104	14	0.9730118145315814
1104	15	0.8812084118932404
1104	16	0.9373925176739704
1104	17	0.8150991188642749
1104	18	0.7142553379609353
1104	19	0.7720660920376403
1104	20	0.8898510926246459
1104	21	0.8880548625113533
1104	22	0.9301311143529603
1104	23	0.8621590705368476
1104	24	0.8549615239576482
1104	25	0.7288754002484339
1104	26	0.7819757976403707
1104	27	0.8564035857170325
1104	28	0.830628317962038
1104	29	0.924407678746519
1104	30	1.0127902255472137
1104	31	1.0106720528223718
1104	32	0.960756584704967
1104	33	0.9509443482957565
1104	34	1.0070387918700803
1104	35	0.9439975995609586
1104	36	0.8761399309755191
1104	37	0.8370328803407503
1104	38	0.8577936375507633
1104	39	0.8491128881874914
1104	40	0.8730861008854447
1104	41	1.0210915040893909
1104	42	0.9825471711278979
1104	43	1.034215553171748
1104	44	0.9801137575032115
1104	45	0.9413641687688781
1104	46	1.0124451909506007
1105	1	0.26473125505523143
1105	2	0.12811628225979632
1105	3	0.4306768330192021
1105	4	0.4014401996432184
1105	5	0.44329910905757686
1105	6	0.4999509848265973
1105	7	0.3225873591864996
1105	8	0.543112887422943
1105	9	0.6539899349494505
1105	10	0.4988132202511082
1105	11	0.489699660281957
1105	12	0.5529238376551717
1105	13	0.6048669849319879
1105	14	0.93890664714759
1105	15	0.7895720643288442
1105	16	0.7478106813219014
1105	17	0.6589190779745024
1105	18	0.4587619050349545
1105	19	0.546585501062367
1105	20	0.664813596941201
1105	21	0.6668643714925722
1105	22	0.5825062278891551
1105	23	0.6432108425943426
1105	24	0.6330339545239454
1105	25	0.6082066009206031
1105	26	0.5871723567208207
1105	27	0.6185562832744722
1105	28	0.7144132401770449
1105	29	0.5603931773648938
1105	30	0.7014433475609252
1105	31	0.7500768854406132
1105	32	0.6828195381661217
1105	33	0.6486392929244715
1105	34	0.6526013990025987
1105	35	0.6803170603284983
1105	36	0.7948612985930481
1105	37	0.80007828655868
1105	38	0.7462720215320076
1105	39	0.7953258074175835
1105	40	0.6085236122837046
1105	41	0.6951735992671502
1105	42	0.68268907323219
1105	43	0.5868225718458149
1105	44	0.7369988668004268
1105	45	0.692011121378215
1105	46	0.7608701936683246
1106	1	0.20408107545509324
1106	2	0.24422179787310583
1106	3	0.25709568979080544
1106	4	0.37506644726454397
1106	5	0.3363634016296615
1106	6	0.3005067049885568
1106	7	0.3493263810034506
1106	8	0.3639420692037234
1106	9	0.33968062228083085
1106	10	0.485471143742501
1106	11	0.41297225049466135
1106	12	0.3643440280745409
1106	13	0.4403707712151004
1106	14	0.7269482497913273
1106	15	0.6128660869665907
1106	16	0.6915963642380163
1106	17	0.6302003002811603
1106	18	0.549162395763588
1106	19	0.5257246073548743
1106	20	0.45763214447672595
1106	21	0.4878794353296243
1106	22	0.48066436066582696
1106	23	0.5870661752929891
1106	24	0.5129212248952157
1106	25	0.5415580297601394
1106	26	0.46173242652289304
1106	27	0.514191571034452
1106	28	0.5294547713793634
1106	29	0.6526630145075742
1106	30	0.7541281451185711
1106	31	0.6584379275178165
1106	32	0.6276510875916017
1106	33	0.67621456004035
1106	34	0.6579525626802578
1106	35	0.466632647300365
1106	36	0.4825256950351289
1106	37	0.36978394568189543
1106	38	0.31390319211642037
1106	39	0.49525886748189407
1106	40	0.5060681103204132
1106	41	0.6656978560916542
1106	42	0.49232009287141665
1106	43	0.5574310354813505
1106	44	0.5554324095981968
1106	45	0.6234094449012346
1106	46	0.4335448819295564
1107	1	0.41517074374939966
1107	2	0.4035480786512551
1107	3	0.41987673205300524
1107	4	0.47219973995193243
1107	5	0.49235019739155206
1107	6	0.4553866592182487
1107	7	0.5002831865000417
1107	8	0.6091819044542603
1107	9	0.7255160064755231
1107	10	0.5723239238699094
1107	11	0.6648898301992574
1107	12	0.7172936097233347
1107	13	0.5985019688121582
1107	14	0.7599428404484811
1107	15	0.6964966050524879
1107	16	0.775179012671245
1107	17	0.6213584005509247
1107	18	0.7024873072101343
1107	19	0.7209188114357516
1107	20	0.7232604866562156
1107	21	0.6260536017073761
1107	22	0.6683064782029788
1107	23	0.6450447595350326
1107	24	0.7834334972250971
1107	25	0.68068209153477
1107	26	0.5913641668709744
1107	27	0.6808652667540329
1107	28	0.7274682384680062
1107	29	0.8256184486539624
1107	30	0.7441149528478732
1107	31	0.7865509598793636
1107	32	0.8082977474829818
1107	33	0.7587738504621413
1107	34	0.6467135035813527
1107	35	0.6911135625565876
1107	36	0.7452729906340565
1107	37	0.7615965481033058
1107	38	0.7351315519154014
1107	39	0.7822677142118479
1107	40	0.8227063255436633
1107	41	0.862809729993181
1107	42	0.8106140645898208
1107	43	0.6710580188547937
1107	44	0.7237511920710702
1107	45	0.7434182315778415
1107	46	0.8281751613160324
1108	1	0.20375715436150443
1108	2	0.1393226883260148
1108	3	0.09676369303129562
1108	4	0.17510001068620795
1108	5	0.19641756311060732
1108	6	0.12743892306220062
1108	7	0.04306633433836993
1108	8	0.2043724902713322
1108	9	0.17930624531248895
1108	10	0.29358143791652225
1108	11	0.21897077506709195
1108	12	0.13406232533571938
1108	13	0.10518463478234708
1108	14	0.16233695936294623
1108	15	0.2480957962170649
1108	16	0.2347360093687243
1108	17	0.1741771142605799
1108	18	0.31137620157448964
1108	19	0.24027217839390147
1108	20	0.2544737753864226
1108	21	0.134091581710436
1108	22	0.20990103986340358
1108	23	0.17101796152295634
1108	24	0.20700746074044218
1108	25	0.2619229765995925
1108	26	0.19390015503740443
1108	27	0.19224403219586605
1108	28	0.2682510627064616
1108	29	0.24326700276208157
1108	30	0.04540710741253662
1108	31	0.13828965215805766
1108	32	0.2950098933471281
1108	33	0.21820142089781314
1108	34	0.2937151125462165
1108	35	0.1914756275618359
1108	36	0.24960065987446
1108	37	0.2724229761930914
1108	38	0.09620542987663327
1108	39	0.21857637319151024
1108	40	0.2171458283825558
1108	41	0.18010946971703845
1108	42	0.18792009014544675
1108	43	0.2948372985521317
1108	44	0.3153059334252504
1108	45	0.23396450211664188
1108	46	0.14830605773970262
1109	1	0.16601184436890648
1109	2	0.09782588236484813
1109	3	0.19211241508026689
1109	4	0.14394279999022785
1109	5	0.17747502467572218
1109	6	0.1775486699197586
1109	7	0.17502002164759034
1109	8	0.15106078691904656
1109	9	0.20031569631785828
1109	10	0.3412852888798099
1109	11	0.2730192236480491
1109	12	0.17897238202886712
1109	13	0.3021146214404169
1109	14	0.4140148999761806
1109	15	0.2897027784683459
1109	16	0.27338171865308425
1109	17	0.2585167959490633
1109	18	0.2479198921769168
1109	19	0.2532016545640161
1109	20	0.0017003791626919451
1109	21	0.2947987980829865
1109	22	0.23847917256718887
1109	23	0.11310555932286803
1109	24	0.24163066185417392
1109	25	0.2794897875824418
1109	26	0.2718957088000806
1109	27	0.14290887431095456
1109	28	0.15858411986098275
1109	29	0.23366173716743788
1109	30	0.3214282376414488
1109	31	0.22735248807160957
1109	32	0.25793416264750846
1109	33	0.1508531776661428
1109	34	0.23012977208537677
1109	35	0.25356781760613956
1109	36	0.16160241410645426
1109	37	0.25673372486312473
1109	38	0.25672255524436594
1109	39	0.29308701466444376
1109	40	0.2817394630648167
1109	41	0.2874590871328948
1109	42	0.279180345867168
1109	43	0.28122589690305944
1109	44	0.2525342334601106
1109	45	0.2697023491082149
1109	46	0.27684783629368326
1110	1	0.09708033594158039
1110	2	0.06603583961461013
1110	3	0.13434479412801892
1110	4	0.1418913883264139
1110	5	0.06770004152261322
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2216	17	-2.441864541545854
2216	18	-2.7404236109075697
2216	19	-2.5022318762961433
2216	20	-2.2467155269376775
2216	21	-2.2517549741831004
2216	22	-2.3038669792476654
2216	23	-2.446575942455226
2216	24	-2.2789659548834607
2216	25	-2.312383455422456
2216	26	-2.2554068582364266
2216	27	-2.4684176917504104
2216	28	-2.45857718216525
2216	29	-2.600650925465686
2216	30	-2.365789612907953
2216	31	-2.3947212883464815
2216	32	-2.45027835736402
2216	33	-2.2454135026131183
2216	34	-2.3040385267629446
2216	35	-2.2451499403211557
2216	36	-2.484007611668744
2216	37	-2.5098394343889723
2216	38	-2.519175801540136
2216	39	-2.479637519631538
2216	40	-2.4538431488810772
2216	41	-2.5522213327848604
2216	42	-2.4540840635409467
2216	43	-2.376206255194326
2216	44	-2.3287820384808313
2216	45	-2.3361029524582264
2216	46	-2.472823509033155
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86506.0
3	2859.0
4	8321.0
5	6362.0
6	5074.0
7	4001.0
8	3584.0
9	3304.0
10	2894.0
11	2988.0
12	2910.0
13	3029.0
14	3129.0
15	3177.0
16	3530.0
17	3850.0
18	4394.0
19	5148.0
20	7079.0
21	9964.0
22	12725.0
23	15039.0
24	16160.0
25	16712.0
26	18372.0
27	21264.0
28	24366.0
29	26557.0
30	29764.0
31	35599.0
32	43820.0
33	55818.0
34	72029.0
35	96615.0
36	133166.0
37	194762.0
38	350023.0
39	1153155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73629073014761	18.45046742896952	13.75471405589013	24.05852778499274
2	15.132952788439692	20.548740643039103	39.25848032700856	25.059826241512635
3	17.98838851656784	25.766859816030863	29.863816828768424	26.380934838632875
4	12.382133136875156	17.139514795488324	38.87056923542166	31.607782832214852
5	13.658281811701217	37.99540275730025	34.82811402480678	13.518201406191762
6	30.997942564635988	37.72731164056656	18.18018857345655	13.094557221340896
7	27.49949860312237	32.47821887752211	22.478415818981055	17.54386670037447
8	26.164637432783678	34.59011458375619	20.606467155590586	18.638780827869546
9	26.576566619065783	15.937628238029076	19.62577907428672	37.860026068618424
10	14.244574765207599	29.043800986234597	34.449763650153194	22.261860598404613
11	33.69571901518017	23.760223371806585	24.44911655678807	18.094941056225178
12	23.053605455519566	25.152358333778796	30.787456356365972	21.006579854335666
13	27.391622914178942	22.601886056102593	26.391883761131712	23.614607268586752
14	22.109813753668035	21.643263456628066	27.629037852550333	28.61788493715357
15	23.196909061605346	29.268820082578827	24.186078478421027	23.3481923773948
16	23.080252840679584	28.60602825748207	25.716897054680192	22.596821847158154
17	22.11409614467923	28.25571272510673	26.96357215834781	22.66661897186623
18	22.037913453789837	28.160295172696525	27.710663234239068	22.09112813927457
19	23.035361167826885	27.721338885226928	27.69380717351811	21.54949277342808
20	23.295393799181188	27.192147569487705	27.586159773697204	21.926298857633903
21	23.439542312905047	27.000250805068195	27.450052009897153	22.11015487212961
22	23.27835987152986	26.81044464960296	27.31083672387481	22.600358754992367
23	23.116602084449507	26.89230049480656	27.201619907694518	22.789477513049412
24	22.677596836381454	27.010169060990652	27.359681548144177	22.952552554483717
25	23.01725901545518	26.89992455887844	27.38524770370811	22.69756872195827
26	22.807902483412565	27.152467737501347	27.409619184350014	22.63001059473607
27	22.9144488220632	27.32246522473265	27.063399533649594	22.699686419554553
28	22.846391970527733	27.11293334415848	27.27064823069138	22.770026454622403
29	23.029338165016224	27.270699374844753	27.20663241723747	22.493330042901547
30	23.12109478307633	27.177496304287185	27.13722328375438	22.564185628882104
31	23.100596539092564	26.867731664320754	27.27444094053982	22.75723085604686
32	22.824551341283676	27.15810559933634	27.26437454255922	22.752968516820758
33	22.995622193533453	26.986210633101877	27.126845455232385	22.891321718132286
34	23.13182378693145	26.989197697095	27.249086410384614	22.629892105588933
35	23.143352895285478	27.078211232909418	27.120735468307185	22.65770040349792
36	23.122738457084964	27.109325999903504	27.058305591740243	22.70962995127129
37	23.213329646106626	26.84519435918626	27.159841277752676	22.781634716954436
38	23.039843459204288	26.96936151260599	27.207484888169642	22.78331014002008
39	23.148813513929767	27.24251316050325	26.965650996040285	22.643022329526698
40	23.206584833272768	26.94303392430465	27.24568831060243	22.604692931820157
41	23.057492417216757	27.068384599731193	27.075261586919087	22.79886139613296
42	23.059766109011587	27.034400772478094	27.123520486072433	22.782312632437886
43	23.039522329303967	26.888515094451886	27.11131766291282	22.96064491333133
44	23.024395264474677	27.11541345388041	27.03375953382888	22.826431747816027
45	23.192550813935583	27.074660312403637	26.824193853802363	22.908595019858414
46	23.160001736719725	26.88944653315366	26.871194895330476	23.079356834796144
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	630.0
1	622.0
2	614.0
3	831.0
4	1048.0
5	1115.5
6	1183.0
7	1183.0
8	1150.0
9	1117.0
10	1345.5
11	1574.0
12	2189.0
13	2804.0
14	4727.5
15	6651.0
16	9273.0
17	11895.0
18	13049.0
19	14203.0
20	14203.0
21	16660.0
22	19117.0
23	23831.0
24	28545.0
25	36004.5
26	43464.0
27	50378.5
28	57293.0
29	65644.0
30	73995.0
31	73995.0
32	82062.0
33	90129.0
34	100200.0
35	110271.0
36	119248.5
37	128226.0
38	135956.0
39	143686.0
40	152474.0
41	161262.0
42	166591.0
43	171920.0
44	171920.0
45	176090.5
46	180261.0
47	183887.0
48	187513.0
49	187946.5
50	188380.0
51	184508.0
52	180636.0
53	172207.5
54	163779.0
55	152863.5
56	141948.0
57	141948.0
58	129913.5
59	117879.0
60	104053.0
61	90227.0
62	77266.0
63	64305.0
64	53451.5
65	42598.0
66	34413.5
67	26229.0
68	20931.0
69	15633.0
70	15633.0
71	12328.0
72	9023.0
73	6941.0
74	4859.0
75	3705.5
76	2552.0
77	1936.0
78	1320.0
79	983.5
80	647.0
81	647.0
82	470.0
83	293.0
84	217.5
85	142.0
86	114.0
87	86.0
88	64.0
89	42.0
90	32.0
91	22.0
92	15.5
93	9.0
94	9.0
95	11.0
96	13.0
97	10.5
98	8.0
99	13.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.192279171350725
2	0.05080285798229858
3	0.062096847771084894
4	0.05405842087515157
5	0.036414073838577934
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	8.03842689593332E-5
16	0.0
17	2.813449413576662E-4
18	5.626898827153324E-4
19	3.617292103169994E-4
20	0.002210567396381663
21	0.0024517202032596627
22	0.0
23	2.4115280687799962E-4
24	4.8230561375599924E-4
25	6.430741516746655E-4
26	8.440348240729984E-4
27	0.026084695277303623
28	6.02882017194999E-4
29	0.0010851876309509983
30	0.0012459561688696644
31	0.0012459561688696644
32	8.842269585526651E-4
33	0.001808646051584997
34	0.0024517202032596627
35	0.0022507595308613295
36	0.03251543679405028
37	0.007274776340819655
38	0.010570531368152315
39	0.05032055236854258
40	0.015795508850508972
41	0.06008724104710156
42	0.06052935452637789
43	0.025923926739384956
44	0.029018721094319282
45	0.02773257279096995
46	0.024155472822279624
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2488049.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.4283701848134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93092018014578	53.89611675119641
2	12.224178156951472	16.485128199440677
3	3.6247769618224805	7.332384084574484
4	1.540076416325117	4.153793708514832
5	0.8159389319639541	2.7508716176333383
6	0.4720701912530124	1.909855416541427
7	0.2974106766049423	1.4037742039323708
8	0.21853020118533995	1.178810824166948
9	0.1494439964453354	0.9069088602792612
>10	0.697484305975367	7.980088620632856
>50	0.023529466476289687	1.0543304855856472
>100	0.0054611403748215425	0.5788000072495814
>500	0.0	0.0
>1k	1.7937447615207759E-4	0.36913722025211654
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4808	0.193243782578237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.01921344796666E-5	0.0
5	0.0	0.0	0.0	4.01921344796666E-5	0.0
6	0.0	0.0	0.0	4.01921344796666E-5	4.01921344796666E-5
7	0.0	0.0	0.0	4.01921344796666E-5	4.01921344796666E-5
8	0.0	0.0	0.0	4.01921344796666E-5	4.01921344796666E-5
9	0.0	0.0	0.0	4.01921344796666E-5	4.01921344796666E-5
10	0.0	0.0	0.0	4.01921344796666E-5	8.03842689593332E-5
11	0.0	0.0	0.0	8.03842689593332E-5	8.03842689593332E-5
12	0.0	0.0	0.0	8.03842689593332E-5	1.205764034389998E-4
13	0.0	0.0	0.0	8.03842689593332E-5	1.205764034389998E-4
14	0.0	0.0	0.0	8.03842689593332E-5	1.205764034389998E-4
15	0.0	0.0	0.0	8.03842689593332E-5	1.607685379186664E-4
16	0.0	0.0	0.0	8.03842689593332E-5	2.00960672398333E-4
17	0.0	0.0	0.0	8.03842689593332E-5	2.00960672398333E-4
18	0.0	0.0	0.0	8.03842689593332E-5	2.00960672398333E-4
19	0.0	0.0	0.0	8.03842689593332E-5	2.00960672398333E-4
20	0.0	0.0	0.0	8.03842689593332E-5	2.411528068779996E-4
21	0.0	0.0	0.0	8.03842689593332E-5	2.813449413576662E-4
22	0.0	0.0	0.0	8.03842689593332E-5	2.813449413576662E-4
23	0.0	0.0	0.0	1.607685379186664E-4	2.813449413576662E-4
24	0.0	0.0	0.0	2.00960672398333E-4	2.813449413576662E-4
25	0.0	0.0	0.0	2.00960672398333E-4	2.813449413576662E-4
26	0.0	0.0	0.0	3.215370758373328E-4	2.813449413576662E-4
27	0.0	0.0	0.0	5.224977482356657E-4	2.813449413576662E-4
28	0.0	0.0	0.0	9.244190930323317E-4	2.813449413576662E-4
29	0.0	0.0	0.0	0.0015674932447069974	2.813449413576662E-4
30	0.0	0.0	0.0	0.0024517202032596627	2.813449413576662E-4
31	0.0	0.0	0.0	0.004300558389324326	2.813449413576662E-4
32	0.0	0.0	0.0	0.006792470727063655	3.215370758373328E-4
33	0.0	0.0	0.0	0.008882461720006318	3.215370758373328E-4
34	0.0	0.0	0.0	0.01241936955421698	3.215370758373328E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3520	0.0	22.776264	1
GGTATCA	1475	0.0	22.285223	1
TATCAAC	4460	0.0	17.414856	2
ATCAACG	4515	0.0	17.060436	3
TCAACGC	4535	0.0	16.806362	4
CAACGCA	4665	0.0	16.377935	5
AACGCAG	4785	0.0	16.003857	6
CGAATCG	115	1.05576E-5	15.655636	37
GTATAGG	490	0.0	14.725553	1
CCGCGAA	85	0.005609154	14.1207695	37
GTCCTAA	385	0.0	14.056207	1
TAATTCG	100	0.0010193053	13.998593	25
CGCACGA	115	1.8468549E-4	13.916121	35
GTGGTAT	655	0.0	13.158073	1
ACGCAGA	5685	0.0	13.15373	7
TAATACG	140	6.741923E-5	12.859987	4
CGCAGAG	5885	0.0	12.638754	8
GTATTAG	400	0.0	12.526944	1
GAGTACT	2715	0.0	12.446102	12
AGAGTAC	5690	0.0	12.298823	11
>>END_MODULE