FastQCFastQC Report
Mon 6 Feb 2017
SRR2077371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488049
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.042.1474151
GTATCAA51900.039.3832361
TCAACGC71150.028.668214
ATCAACG71650.028.4681533
TATCAAC71400.028.4240442
CAACGCA72150.028.0291825
AACGCAG74400.027.2713096
ACGCAGA86200.023.1374027
CGCAGAG88050.022.4836588
AGAGTAC91050.020.49323311
GAGTACT59400.020.148212
AGTACTT62300.020.04073713
CAGAGTA95550.019.81646210
GTACATG66000.019.7890151
GCAGAGT98450.019.4429329
GTACTTT63550.018.77554914
TACATGG66450.018.4499322
CTCGTAT3200.018.31986639
TACTTTT70200.016.9481715
TCGTATG3700.016.7762240
Produced by FastQC (version 0.11.5)