FastQCFastQC Report
Mon 6 Feb 2017
SRR2077370_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077370_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819998
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67520.23943279392396732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.10964546783366513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.10666674231683852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.024.7525751
GTATCAA41650.024.550511
TATCAAC56950.017.543882
TCAACGC57050.017.5348384
CAACGCA57750.017.3872625
ATCAACG57250.017.3715113
CCTATAC2850.016.85093
AACGCAG60600.016.7922556
ACGCAGA70600.014.1588887
GCGTATC850.005526143614.1526481
GTATAGC5350.013.8660671
CGAATCG1303.3598328E-513.85018837
CGCAGAG72950.013.483538
GTATTAT5900.013.2531151
GTACATG73350.012.8743761
GTACTAG2201.8037099E-812.7588271
CGCACGA1753.0655447E-612.57486935
AGAGTAC71450.012.56342211
TACATGG74250.012.4314932
CTTATAC4850.012.3775943
Produced by FastQC (version 0.11.5)