FastQCFastQC Report
Mon 6 Feb 2017
SRR2077370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819998
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.042.1315041
GTATCAA54250.039.1814271
TATCAAC77000.027.3687082
ATCAACG77250.027.1889423
TCAACGC77200.027.1611714
CAACGCA78850.026.6343845
AACGCAG81300.025.9591676
ACGCAGA95850.021.921687
CGCAGAG97600.021.4833588
GGACCGT2800.021.0404456
GTACATG74700.020.6101821
AGAGTAC98550.020.05289811
GAGTACT66300.019.24719212
TACATGG76850.019.2135282
CAGAGTA106300.019.0772410
CGAATCG1651.8399805E-618.80778337
GCAGAGT108800.018.7329859
AGTACTT70600.018.31916813
ACATGGG78900.018.1422523
GTACTTT71600.017.62635614
Produced by FastQC (version 0.11.5)