FastQCFastQC Report
Mon 6 Feb 2017
SRR2077369_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077369_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363078
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34000.14388014276295577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29250.023.7799151
GGTATCA11150.023.1911091
CGAATCG754.7100984E-621.34061637
TATCAAC38950.017.8343732
GCGATAC1256.8477675E-817.5944614
ATCAACG39150.017.5812993
TCAACGC39700.017.2836674
CAACGCA40200.016.9673735
AACGCAG41400.016.46726
GTATAGT3300.015.7930531
TGCGATA1058.5588996E-515.23265413
GTATAGG5000.014.031521
ACGCAGA48750.013.6973817
TACCGAT1903.7956852E-813.6790347
TTATACT6100.013.4457894
TATACAG8350.013.4149135
GTCATAC2109.43146E-913.3633511
CGCAGAG49950.013.3282928
GTATTAA7700.013.2765771
GTACATG56000.013.06506251
Produced by FastQC (version 0.11.5)