FastQCFastQC Report
Mon 6 Feb 2017
SRR2077369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363078
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40300.038.299191
GGTATCA18300.038.096021
TCAACGC54900.027.4050254
TATCAAC55350.027.2483312
ATCAACG55850.026.9394443
CAACGCA55650.026.7842315
AACGCAG58200.025.6096066
ACGCAGA68600.021.6789367
CGCAGAG71350.020.7853348
AGAGTAC71300.019.68860611
CAGAGTA74600.019.00179910
GAGTACT42650.018.60025412
GCAGAGT79650.018.1434549
TGTACCG3250.018.1318235
GTACATG59400.017.5757851
AGTACTT46600.016.80157713
TACATGG61300.016.572022
GTACTTT47300.016.18421614
TATGCCG3502.3646862E-1115.76179843
GTACCGT2201.544844E-615.7555926
Produced by FastQC (version 0.11.5)