FastQCFastQC Report
Mon 6 Feb 2017
SRR2077368_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077368_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325979
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57620.24772364668812574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26500.11393052129877355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25970.11165191087279806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.025.4884361
GTATCAA37250.023.6345251
TATCAAC49650.017.461632
ATCAACG50000.017.2477993
TCAACGC50300.017.1430844
CAACGCA51350.016.8286115
AACGCAG53100.016.26426
CGCAGAG60850.013.897078
ACGCAGA61650.013.8464457
CGAACTC3200.013.74468720
TAGCCCT7450.013.4274454
ACCGTGC2701.0913936E-1113.3276098
TGCGAAA1202.6634365E-413.32760812
AGAGTAC59500.013.20441211
GTATTAC2901.8189894E-1213.1390591
GTATAGA5400.012.9981921
GTGTAGC6950.012.9848331
GTATAAT7150.012.9021011
TATACTC4050.012.8417235
GTACATG60350.012.793581
Produced by FastQC (version 0.11.5)