FastQCFastQC Report
Mon 6 Feb 2017
SRR2077368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325979
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.040.467291
GTATCAA49150.036.826661
TATCAAC67550.026.5820432
ATCAACG67800.026.4300423
TCAACGC67800.026.2772434
CAACGCA69450.025.6523935
AACGCAG72800.024.6126636
ACGCAGA84150.021.11847
CGCAGAG86350.020.610568
GTACATG62950.020.0503081
TACATGG65200.019.2461952
AGAGTAC87300.019.12283111
GAGTACT59000.019.05840912
AGTACTT62100.018.38473913
ACATGGG66000.018.2756273
CAGAGTA93400.018.16895510
GCAGAGT96600.018.1379839
GTACTTT63850.017.49781414
GTCGTAA1452.6208218E-416.7396131
TAATCGG1653.8602884E-516.71918315
Produced by FastQC (version 0.11.5)