FastQCFastQC Report
Mon 6 Feb 2017
SRR2077367_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077367_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500160
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56510.22602553436580058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27270.10907301932676308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26110.10443331626775886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.024.1289791
GTATCAA36400.023.3475441
TATCAAC48300.017.283282
ATCAACG47750.017.2225323
CGCACGA1401.3258614E-817.14760835
TCAACGC48750.016.866554
CAACGCA49100.016.742975
GTGCCGA1704.947651E-1016.46461111
AACGCAG50700.016.3254326
ACGCAGA59400.013.698737
TAAGCGT1354.7901107E-513.33729654
CGCAGAG61000.013.2738698
GTATAAA6950.013.26631
GTACATG63600.013.1418261
AGAGTAC58950.012.88760311
TACATGG64000.012.85582
GAGTACT30650.012.78491712
GACGGTC1100.002094688612.7226527
CTTACAC5400.012.59835153
GCACCGT2701.5825208E-1012.5880216
Produced by FastQC (version 0.11.5)