FastQCFastQC Report
Mon 6 Feb 2017
SRR2077367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500160
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22050.040.2585331
GTATCAA50400.039.4230961
TATCAAC70000.028.0237752
TCAACGC70150.027.8133984
ATCAACG70500.027.6256073
CAACGCA71100.027.2457085
AACGCAG74150.026.2646926
ACGCAGA84750.022.5130187
CGCAGAG88200.021.4669118
AGAGTAC87650.020.6974611
GAGTACT58950.020.36004312
GTACATG66050.020.0547451
CAGAGTA93600.019.63929610
GCAGAGT97300.019.1402119
TACATGG68050.018.9971732
AGTACTT64550.018.70057713
TATACCG1501.6313414E-518.482245
ACATGGG70250.017.9564173
GTACTTT66700.017.73228314
CGAATCG2208.639108E-817.24012237
Produced by FastQC (version 0.11.5)