FastQCFastQC Report
Mon 6 Feb 2017
SRR2077366_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077366_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784609
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57320.2058457758342374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.025.6436371
GTATCAA39800.023.7724081
TCAACGC54450.017.1555164
TATCAAC54300.017.066292
CAACGCA55250.016.9405755
ATCAACG55050.016.8967743
AACGCAG58100.016.1387886
ACGCAGA66650.014.0384747
ATTATAC7050.013.9032133
TATACCG1303.3701548E-513.8456635
CGCAGAG68950.013.4831978
AGAGTAC65850.013.05530111
GTATTAT6950.012.9790211
GTATTAA9200.012.8551861
GAGTACT33200.012.70628512
CAGAGTA68850.012.60259510
GTATAGG5650.012.4174891
GTATATA9550.012.3840551
GCAGAGT72100.012.339549
TAGACGT1150.002916929312.173485
Produced by FastQC (version 0.11.5)