FastQCFastQC Report
Mon 6 Feb 2017
SRR2077366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784609
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25400.040.66251
GTATCAA58950.038.098051
TATCAAC80900.027.6660162
ATCAACG81550.027.3995553
TCAACGC81550.027.3145964
CAACGCA82300.027.0221235
AACGCAG85150.026.1583656
ACGCAGA98600.022.321437
CGCAGAG102200.021.5316758
GAGTACT71300.020.80150612
AGAGTAC103100.020.77537211
AGTACTT75100.019.84082213
CAGAGTA109850.019.56122410
GCAGAGT113800.019.1550319
GTACTTT77650.018.8739114
GTACATG71600.018.394251
TACATGG72950.017.3352532
TACTTTT86700.016.82455315
ACATGGG75950.016.4203553
ACTTTTT92150.016.05490116
Produced by FastQC (version 0.11.5)