FastQCFastQC Report
Mon 6 Feb 2017
SRR2077365_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077365_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2495332
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33340.13360947561286432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31950.024.720641
GGTATCA12150.024.2535571
ATCAACG42250.018.2311463
TCAACGC42700.018.0811564
TATCAAC43400.018.079822
CAACGCA43600.017.7971745
AACGCAG45300.017.1217386
TGCGATA1806.184564E-1116.6590613
GCGATAC1859.458745E-1116.20881714
CGATACT2052.7284841E-1115.6035715
TAACACT4250.014.1188434
ACGCAGA54550.014.0718377
CGCACGA2004.8767106E-914.005959535
CGCAGAG55550.013.7825348
GTATAGA3450.013.3642041
TCGCTAA900.00822566213.32724814
CGTGCCG2109.802534E-913.326714510
GAGTACT23200.013.26980412
GTATTAT4550.013.2173441
AGAGTAC54500.013.02131711
Produced by FastQC (version 0.11.5)