FastQCFastQC Report
Mon 6 Feb 2017
SRR2077365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2495332
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.040.5780531
GTATCAA46700.036.4562531
TATCAAC63900.026.7399732
ATCAACG63800.026.5075933
TCAACGC64200.026.2889864
CAACGCA64150.026.1458265
AACGCAG67950.024.7331666
ACGCAGA77800.021.514267
CGCAGAG80550.020.8121668
GTACATG65800.020.4989281
GAGTACT52550.019.955612
AGAGTAC81600.019.48833511
TACATGG66950.019.206062
CAGAGTA88150.018.39207610
AGTACTT58250.018.18052513
GCAGAGT91900.018.0916469
ACATGGG69200.018.0789133
GTACTTT57700.017.92778414
CATGGGG52450.016.1882744
TACTTTT64000.016.1091415
Produced by FastQC (version 0.11.5)