FastQCFastQC Report
Mon 6 Feb 2017
SRR2077364_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077364_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742262
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52060.18984327536902015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17250.026.6103151
GTATCAA38700.022.6864621
CGAATCG1204.2582542E-818.34095237
TGCGATA1401.3364115E-817.13684713
GTATAGG3850.016.6606861
TATCAAC53050.016.1851632
TATAGCG750.00244491615.9981835
ATCAACG53000.015.9665673
TCAACGC53200.015.827584
GCGATAC1402.4909787E-715.70906414
CAACGCA54000.015.4797235
AACGCAG55750.015.2054316
GTATAAT6000.015.0336671
AGTACCG3950.014.1756055
GTATAGC4900.013.0905391
GTATAAA7350.013.0905391
GTACCGA2454.5838533E-1013.0566466
TACCGAT2151.3604222E-813.0186927
ACGCAGA64900.012.9076477
GTACATG76400.012.6723581
Produced by FastQC (version 0.11.5)