FastQCFastQC Report
Mon 6 Feb 2017
SRR2077364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742262
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.037.6913261
GTATCAA52050.037.488651
TATCAAC72350.026.9596922
TCAACGC71850.026.9027564
CAACGCA72400.026.8409425
ATCAACG72700.026.77933
AACGCAG75650.025.8251956
ACGCAGA90200.021.4225757
CGCAGAG93500.020.5091368
AGAGTAC92350.019.60822711
GAGTACT53550.018.80785412
GTACATG80450.018.6540871
CAGAGTA100350.018.49119210
GCAGAGT103850.018.133369
AGTACTT57850.017.58873213
TACATGG83800.017.4467092
ACATGGG84350.016.7560793
GTACTTT59900.016.75278514
CGAATCG2301.4328543E-716.49186737
TACCGAT3401.4551915E-1116.2379517
Produced by FastQC (version 0.11.5)