FastQCFastQC Report
Mon 6 Feb 2017
SRR2077363_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077363_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784464
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39170.14067339351487396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.023.622981
GTATCAA36050.023.0653021
TCAACGC47550.017.2043974
ATCAACG47800.017.158113
CAACGCA47700.017.1481385
TATCAAC48250.017.0057262
AACGCAG49900.016.6263076
CGAATCG1505.397069E-714.67317737
ACGCAGA57300.014.23487957
CGTATAC1000.001017105214.0026923
GTATATA6900.013.9382581
CGCAGAG59300.013.5187938
GTATACG900.008103630513.3574971
ACCGATC2851.8189894E-1213.3277558
GTACTAA3050.013.1385221
TAGACTG5450.012.8435075
TATACTG6550.012.8238975
GTATTAT5650.012.7664581
GTACATG72000.012.7452781
CGTATTA1100.002095099312.722404528
Produced by FastQC (version 0.11.5)