FastQCFastQC Report
Mon 6 Feb 2017
SRR2077363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784464
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.037.102751
GTATCAA46100.036.9174231
TATCAAC63900.026.51422
ATCAACG63550.026.4378553
TCAACGC63950.026.2729644
CAACGCA64750.025.8925175
AACGCAG68550.024.5578126
CGAATCG2005.2750693E-1122.41241637
GTACATG73800.021.7927681
ACGCAGA78100.021.2979627
TACATGG75950.020.6652662
CGCAGAG80600.020.4144698
GAGTACT47250.019.56605712
AGAGTAC80800.019.34003311
ACATGGG81400.019.1082953
CATGGGG59300.018.5187824
CAGAGTA86550.018.45340310
GTATAAT7250.018.1675511
AGTACTT52200.017.7767413
GCAGAGT92100.017.6407099
Produced by FastQC (version 0.11.5)