FastQCFastQC Report
Mon 6 Feb 2017
SRR2077362_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077362_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015664
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35010.1736896625628081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.023.885021
GTATCAA25750.022.4278981
TATCAAC34250.016.487182
TCAACGC33650.016.1101634
ATCAACG34250.015.8902893
CAACGCA34000.015.881155
TACGTAG1151.0590342E-515.65021326
AACGCAG35200.015.3351826
ATACGTA1352.9344483E-614.81295925
GGACCGT1101.2721383E-414.5400246
ATTATAC4100.013.6646233
TTAGGGT2657.2759576E-1213.5875864
GTCTTAG4400.013.2165671
TATACTG4550.013.1858155
CCTTATA3500.013.1588412
GTATAAA6000.013.0342691
ACGCAGA41350.012.9576777
CGCAGAG43000.012.2744868
GTGTAAG5650.012.0671241
CTAATAC3650.012.0601663
Produced by FastQC (version 0.11.5)