FastQCFastQC Report
Mon 6 Feb 2017
SRR2077362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015664
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36750.039.4633641
GGTATCA15200.037.434791
TATCAAC49000.028.8064122
TCAACGC50500.027.880714
CAACGCA50750.027.5383665
ATCAACG51050.027.5117133
AACGCAG52950.026.459026
ACGCAGA61450.022.5663057
CGCAGAG62450.022.2513568
GAGTACT41850.021.1770112
AGAGTAC63550.020.7288311
CAGAGTA67000.019.9687810
TACATGG48800.019.4724962
AGTACTT45450.019.4237513
GTACATG49150.019.3420351
GTACTTT45850.019.17717714
GCAGAGT71350.019.0413139
CGAATCG1451.2720448E-519.02323237
GTGCCGA2759.094947E-1218.80985811
CGTGCCG2406.1299943E-1018.67784510
Produced by FastQC (version 0.11.5)