FastQCFastQC Report
Mon 6 Feb 2017
SRR2077361_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077361_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450998
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59970.24467584224874928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28670.11697275966769455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.11028160773693002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.026.1254181
GTATCAA39600.023.2346021
TATCAAC52650.017.2228532
ATCAACG53050.017.1202343
TCAACGC53500.017.0122364
CAACGCA53650.016.9253125
AACGCAG55400.016.420746
ATACCGT1453.7080827E-715.16756256
ACGCAGA64500.014.0730167
CGCAGAG66600.013.4491518
GAGTACT30900.012.8761412
GTACATG71150.012.790831
TACATGG70150.012.75513652
AGAGTAC63950.012.69335911
ACCCGTG1601.7006803E-512.4960037
CAGAGTA67600.012.45163710
GCAGAGT68750.012.4469289
GTATAAT6700.012.2666731
ACCGTGC2955.4569682E-1112.1994878
ACATGGG73450.012.1746073
Produced by FastQC (version 0.11.5)