FastQCFastQC Report
Mon 6 Feb 2017
SRR2077361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450998
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24100.043.5719031
GTATCAA51250.036.448181
TCAACGC72250.025.8462964
TATCAAC72750.025.622612
CAACGCA73550.025.481585
ATCAACG73150.025.4804133
CCTATCG700.001336689124.6352218
AACGCAG77100.024.4425986
TATGCCG3550.021.37016743
ACGCAGA88550.021.1180957
TCGATCG1154.622186E-520.99006741
CGCAGAG90500.020.6173658
GTACATG64600.020.171411
AGAGTAC91900.019.40376911
GAGTACT63800.019.19193312
TACATGG65850.019.099632
CTCGTAT3450.018.9910139
CAGAGTA97300.018.46830710
GCAGAGT100250.018.2685249
AGTACTT67500.017.98663513
Produced by FastQC (version 0.11.5)