FastQCFastQC Report
Mon 6 Feb 2017
SRR2077360_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077360_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618918
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58090.221809159355123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28090.10725803557041495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26910.10275235803488311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39500.023.661851
GGTATCA16100.022.2990841
TATCAAC51550.017.8672492
ATCAACG52400.017.568353
TCAACGC52400.017.5278134
CAACGCA53000.017.2118455
AACGCAG56100.016.287386
ACGCAGA64250.013.97240457
CGCAGAG66100.013.520858
GTACATG66400.012.7166841
AGAGTAC64200.012.70641511
CAGAGTA67650.012.53129610
CTAGACA4400.012.2788424
ACCGTCG1150.002922725212.1702128
GCAGAGT70800.012.1431999
GAGTACT32150.012.0649912
TACATGG69100.012.0253742
TATACTG6750.011.8548135
GACCGCG1200.004018540511.6631197
ACATGGG70250.011.4805613
Produced by FastQC (version 0.11.5)