FastQCFastQC Report
Mon 6 Feb 2017
SRR2077360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618918
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.042.191681
GTATCAA52750.037.7577931
TATCAAC74650.026.253912
ATCAACG74600.025.9450243
TCAACGC74550.025.869374
CAACGCA74350.025.8441625
AACGCAG77750.024.8468766
ACGCAGA89750.021.2983597
CGCAGAG94100.020.1929868
CGCACGA2102.3701432E-919.70270535
GAGTACT62050.019.67508312
AGAGTAC94500.019.19592511
TGCGACG900.00575584219.15540756
GTACATG72750.018.9354111
AGTACTT64850.018.87876113
GTACTTT65950.018.7171814
CAGAGTA100000.018.34672210
TACATGG73600.018.3335742
GTGCGAC1150.001115126917.98942655
GCAGAGT104100.017.8556869
Produced by FastQC (version 0.11.5)