FastQCFastQC Report
Mon 6 Feb 2017
SRR2077359_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077359_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075612
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49170.23689398596654868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22930.11047344108629166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22860.11013619115711414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28150.022.5035551
GGTATCA11900.022.4051131
TATACCG901.0212534E-619.9993385
ATACCGC659.3951833E-418.4542566
TATCAAC38050.016.569392
TCAACGC38050.016.5064374
ATCAACG38050.016.2455543
CAACGCA38850.016.0612435
TATACTG5250.015.9994715
TACGACT1151.0566895E-515.6539184
AACGCAG40500.015.5000556
ATTATAC4150.014.4611853
TCTATAC3300.013.9426283
GAGTACT23150.013.5586912
GTATTAT4400.013.21258451
ACGCAGA46500.013.1990937
CGCAGAG47450.012.8084348
TACGATA1253.7502561E-412.7986522
GTACATG51650.012.7693281
GTGTAAG4950.012.5544861
Produced by FastQC (version 0.11.5)