FastQCFastQC Report
Mon 6 Feb 2017
SRR2077359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075612
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.042.514971
GTATCAA43750.038.1799771
ATCAACG60550.027.3451713
TCAACGC60600.027.2082884
CAACGCA61400.026.8531325
TATCAAC61650.026.7481272
AACGCAG64850.025.5301486
ACGCAGA74000.022.24267
CGCAGAG76650.021.3282748
TGCGATA1304.935655E-621.2254613
GAGTACT51650.020.50116312
AGAGTAC76400.020.45103611
GCGATAC1356.8553272E-620.434914
CAGAGTA79950.019.71504410
GCAGAGT83150.019.2462129
AGTACTT55400.019.1757113
GTACATG53500.018.8497621
GTACTTT57000.018.45190814
TACATGG54250.018.3274332
ACATGGG56950.017.1522983
Produced by FastQC (version 0.11.5)