FastQCFastQC Report
Mon 6 Feb 2017
SRR2077358_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077358_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281106
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56090.24588949395600207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24930.10928909046751883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24720.10836848441063238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.023.7819941
GTATCAA36300.023.1923561
TATCAAC48050.017.3301092
ATCAACG48900.016.8967933
TCAACGC49050.016.8429054
CAACGCA49600.016.652125
AACGCAG50600.016.357176
CGTGCGG1201.5888343E-514.99407410
TAACGGA1101.2724902E-414.53970718
GTATTAG3400.014.1492861
ACGCAGA58750.013.8498457
CCGTGCG1202.662628E-413.3280669
CGCAGAG60900.013.2624098
GTATAAC2201.808985E-812.7557961
CCTAATA3201.8189894E-1212.5106932
AGAGTAC60500.012.49093111
CAGAGTA62600.012.32739510
ACCGCCC1957.698309E-712.302838
GAGTACT29150.012.20786912
GCAGAGT65400.011.9524629
Produced by FastQC (version 0.11.5)