FastQCFastQC Report
Mon 6 Feb 2017
SRR2077358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281106
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.040.7661321
GTATCAA51600.038.7655221
ATCAACG71050.027.944093
TCAACGC71300.027.8454974
TATCAAC70800.027.8005122
CAACGCA71950.027.5439625
AACGCAG74600.026.6113826
ACGCAGA84750.023.0814087
CGCAGAG86850.022.4369748
GAGTACT60100.022.20962112
AGAGTAC84600.021.7300911
AGTACTT64350.021.06437913
CAGAGTA90900.020.52669310
GTACTTT66200.020.36660814
GCAGAGT95000.019.8586339
TACTTTT73600.018.55476215
GTACATG56250.018.4275761
ACTTTTT78000.017.86256616
TACATGG58450.017.6079852
TATACGA1200.001435997717.3233225
Produced by FastQC (version 0.11.5)