FastQCFastQC Report
Mon 6 Feb 2017
SRR2077357_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077357_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505097
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50710.20242729123862271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14650.024.7629241
GTATCAA34950.023.5697841
TATCAAC46200.017.5891912
ATCAACG46800.017.269183
TCAACGC47800.016.8623474
CAACGCA49750.016.2403165
AACGCAG51850.015.7324446
AGTCGCG850.00562689114.11405318
GTATAAT5600.013.6005531
ACGCAGA58450.013.5797197
CTTATAC4900.013.4726843
CGCAGAG60150.013.1294428
GTATAAA8000.012.7773611
GAGTACT27800.012.58571912
GTGTAGC7200.012.5268241
AGAGTAC58250.012.25341211
TAGACAG7350.011.9721325
GTATTAA7200.011.9700771
ACCGTGC3700.011.8879168
GTCCTAC2551.1150405E-811.7899531
Produced by FastQC (version 0.11.5)