FastQCFastQC Report
Mon 6 Feb 2017
SRR2077357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505097
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48150.038.9486921
GGTATCA21800.036.7322351
TCAACGC65600.027.8860324
ATCAACG66300.027.5393543
TATCAAC67100.027.471942
CAACGCA67000.027.1465725
AACGCAG69500.026.1191836
ACGCAGA80000.022.6017467
CGCAGAG83250.021.6666938
AGAGTAC84100.020.79197911
GAGTACT54800.020.32852412
CAGAGTA89300.019.81297710
AGTACTT57200.019.47557813
GTACATG65600.019.3934081
GCAGAGT92500.019.3135999
GTACTTT58500.018.8032114
TACATGG67950.018.25552
ACATGGG70250.016.9162643
GTATTAG4800.016.6103381
ATACCGT2351.7362254E-716.2157826
Produced by FastQC (version 0.11.5)