FastQCFastQC Report
Mon 6 Feb 2017
SRR2077356_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077356_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950274
Sequences flagged as poor quality0
Sequence length46
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75820.38876588622931957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35740.1832563014222617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34480.17679567076215957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.026.4204541
GTATCAA47150.023.5989321
TATCAAC61650.017.7280142
ATCAACG62500.017.2503223
TCAACGC63650.016.9356124
CAACGCA64450.016.6939355
AACGCAG65600.016.3962366
CGGATAT800.00376334614.99509716
ACGCAGA74200.014.3072567
AGACCGT2106.384653E-1014.2803136
CGCAGAG75050.013.9853828
GTGGTAT6450.013.3656421
GTATAGG4550.013.2187661
TATACTG5750.013.2164595
GTCCTAA3650.013.182551
GTACATG77850.013.056611
GCAGAGT77450.012.9066749
GTCTTAG4200.012.8882971
AGAGTAC74400.012.84460411
CAGAGTA76850.012.82533710
Produced by FastQC (version 0.11.5)