FastQCFastQC Report
Mon 6 Feb 2017
SRR2077356_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077356_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950274
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.13136615675540975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.043.3475951
GTATCAA63550.038.7575231
ATCAACG88000.027.8141963
TATCAAC88850.027.5502362
TCAACGC89000.027.5406284
CAACGCA91400.026.778155
AACGCAG94600.026.0555656
ACGCAGA107800.022.7274687
CGCAGAG110050.022.0314358
GTGGTAT9900.021.7254981
GAGTACT74000.021.67254312
TAGTCGG1455.3266376E-721.40132340
AGAGTAC109300.021.33119411
TCTAACG1154.4316766E-521.1046542
AGTACTT78200.020.77317213
CAGAGTA114650.020.63662710
GCAGAGT116300.020.6107469
GTACATG75500.020.4927921
GTACTTT80850.019.57486714
TACATGG78150.019.4322532
Produced by FastQC (version 0.11.5)