FastQCFastQC Report
Mon 6 Feb 2017
SRR2077355_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077355_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048466
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39170.19121625645727094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29150.024.6976071
GGTATCA11750.023.3820021
TATCAAC39150.018.407472
ATCAACG38800.018.0967943
TCAACGC39300.017.9652884
CAACGCA40250.017.489865
AACGCAG42000.016.944556
CGTGCCG1402.4937435E-715.707026510
GCGATAC1606.919254E-814.99307114
GAGTACT22250.014.73476312
ACGCAGA47850.014.5805147
CGCAGAG48950.014.1711848
TGCGATA1701.452936E-714.11112613
AGAGTAC47650.013.76069311
TACCGAT1601.1293578E-613.7436487
GTACTTT22950.013.67559614
GTGTACG1508.482668E-613.3692711
GCGAATC900.00822530113.32717513
GTCTTAG3500.013.178281
GTATAAT6250.013.1553621
Produced by FastQC (version 0.11.5)