FastQCFastQC Report
Mon 6 Feb 2017
SRR2077355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048466
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18900.040.3684921
GTATCAA37950.036.5535771
TCAACGC52450.026.231814
ATCAACG52600.026.0904753
TATCAAC52750.026.0864642
CAACGCA53300.025.7465615
AACGCAG56300.024.742126
CGAATCG2700.022.98637837
ACGCAGA65050.021.005257
CGCAGAG67150.020.3408958
GTACCGA3500.019.8007326
TACCGAT3550.019.4393447
CCGTTAT3450.018.9878266
AGAGTAC67250.018.9771211
CAACGCT3700.018.6371557
GAGTACT41800.018.48385812
TACAACG3750.018.39224616
TCCGTTA3400.018.25299865
TAACGCA1351.524012E-417.9699764
GTACGCT2701.5279511E-1017.87785760
Produced by FastQC (version 0.11.5)