FastQCFastQC Report
Mon 6 Feb 2017
SRR2077354_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077354_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416072
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58540.24229410381809816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28800.11920174564334177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28260.11696671291252911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14000.027.0567131
GTATCAA37300.024.6629581
TATACCG751.13879614E-418.6667655
TATCAAC52150.017.3863722
ATCAACG53250.016.7940353
TCAACGC53750.016.5988714
CAACGCA54850.016.2626195
AACGCAG56950.015.6567846
CGTGCGC1801.8655555E-814.43883910
ACGCAGA66350.013.408517
GAGTACT30750.013.39372912
AACCGCG900.008179205513.33864938
CCGATAA900.00822184413.3281599
ATTATAC5900.013.2245133
GTACTAA3050.013.14230251
CGCAGAG67850.012.9647538
GTACATG68950.012.440871
GCACCGT2252.568595E-812.4396166
AGTACTT33100.012.32201213
CGAATCG1305.1990943E-412.31234537
Produced by FastQC (version 0.11.5)