##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077354_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2416072
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.057189934737043	31.0	31.0	34.0	26.0	34.0
2	30.169158452231557	31.0	31.0	34.0	26.0	34.0
3	30.292426301865177	33.0	31.0	34.0	26.0	34.0
4	33.67716897509677	37.0	35.0	37.0	28.0	37.0
5	33.74303621746372	37.0	35.0	37.0	28.0	37.0
6	33.793373293511124	37.0	35.0	37.0	29.0	37.0
7	33.70402082388273	37.0	35.0	37.0	28.0	37.0
8	33.61640505746517	37.0	35.0	37.0	28.0	37.0
9	35.106072170034665	39.0	37.0	39.0	28.0	39.0
10	35.04110390749945	39.0	35.0	39.0	27.0	39.0
11	35.05284155439076	39.0	37.0	39.0	27.0	39.0
12	34.975153886142465	39.0	35.0	39.0	27.0	39.0
13	34.932828988540074	39.0	35.0	39.0	27.0	39.0
14	35.97751350125328	40.0	37.0	41.0	27.0	41.0
15	36.106785311033775	40.0	37.0	41.0	27.0	41.0
16	36.077873507080916	40.0	37.0	41.0	27.0	41.0
17	36.09986581525716	40.0	37.0	41.0	27.0	41.0
18	36.06471744219543	40.0	37.0	41.0	27.0	41.0
19	36.15860454489767	40.0	37.0	41.0	27.0	41.0
20	36.15548212139373	40.0	37.0	41.0	27.0	41.0
21	36.1658361174667	40.0	37.0	41.0	27.0	41.0
22	36.116676158657526	40.0	37.0	41.0	27.0	41.0
23	36.06907078928111	40.0	37.0	41.0	27.0	41.0
24	36.09252249105159	40.0	37.0	41.0	27.0	41.0
25	36.03679236380373	40.0	37.0	41.0	27.0	41.0
26	36.064316791883684	40.0	37.0	41.0	27.0	41.0
27	36.03950089235751	40.0	37.0	41.0	27.0	41.0
28	35.97474785519637	40.0	37.0	41.0	27.0	41.0
29	35.940364773897464	40.0	36.0	41.0	27.0	41.0
30	35.853211328139224	40.0	36.0	41.0	27.0	41.0
31	35.819697840130594	40.0	36.0	41.0	26.0	41.0
32	35.789666036442625	40.0	36.0	41.0	26.0	41.0
33	35.74345342357347	40.0	36.0	41.0	26.0	41.0
34	35.69094795188223	40.0	36.0	41.0	26.0	41.0
35	35.6614430364658	40.0	36.0	41.0	25.0	41.0
36	35.60140881563132	40.0	36.0	41.0	25.0	41.0
37	35.512212798294094	40.0	36.0	41.0	25.0	41.0
38	35.47434554930482	40.0	36.0	41.0	25.0	41.0
39	35.39048670734978	40.0	35.0	41.0	25.0	41.0
40	35.365988679145325	40.0	35.0	41.0	25.0	41.0
41	35.225898483157785	40.0	35.0	41.0	24.0	41.0
42	35.17409539119695	40.0	35.0	41.0	24.0	41.0
43	35.06784690191352	40.0	35.0	41.0	23.0	41.0
44	34.98508984831578	40.0	35.0	41.0	23.0	41.0
45	34.8784365697711	40.0	35.0	41.0	22.0	41.0
46	34.76699080159863	40.0	35.0	41.0	20.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32270691823620723
1101	2	0.3391883130676163
1101	3	0.4173233646667249
1101	4	0.4740331600362353
1101	5	0.3820414788897324
1101	6	0.31004310587168504
1101	7	0.3496125252674247
1101	8	0.4991690137029181
1101	9	0.5521991419527765
1101	10	0.5304670805631773
1101	11	0.5599602683843017
1101	12	0.5558248317339718
1101	13	0.5593442124048167
1101	14	0.7951040508035376
1101	15	0.734065596155844
1101	16	0.6337358973876235
1101	17	0.55598416536548
1101	18	0.6935130643190419
1101	19	0.6390201296407625
1101	20	0.45430744454240113
1101	21	0.5733641205417186
1101	22	0.6814352820420382
1101	23	0.6573700364943065
1101	24	0.5759638248214785
1101	25	0.6282536417173503
1101	26	0.7637398508783733
1101	27	0.7350733330785744
1101	28	0.7202681232233559
1101	29	0.6334841048130357
1101	30	0.6513256482193626
1101	31	0.7278833454233862
1101	32	0.719136762686837
1101	33	0.790533787821623
1101	34	0.6980827822598954
1101	35	0.7846135370570053
1101	36	0.6682645163072891
1101	37	0.6921092884058169
1101	38	0.6708183942817243
1101	39	0.7236506040107287
1101	40	0.7597281872907473
1101	41	0.8490839407848085
1101	42	0.6463947571827262
1101	43	0.6124898978029663
1101	44	0.6462244029011401
1101	45	0.6331229742327551
1101	46	0.6090485839320863
1102	1	0.705749883136594
1102	2	0.7863261147184986
1102	3	0.8347352579384406
1102	4	0.704922364053651
1102	5	0.748303598746439
1102	6	0.6922363770529358
1102	7	0.7026292179422242
1102	8	0.7747108052286791
1102	9	0.900299277943887
1102	10	0.9017528150020908
1102	11	0.830034919748293
1102	12	0.9017586540736815
1102	13	0.884094360497393
1102	14	1.221781759226289
1102	15	1.1663588716160689
1102	16	1.139770335765732
1102	17	1.1319317573065675
1102	18	1.1777579191883163
1102	19	1.1194777591164708
1102	20	1.1358330956483158
1102	21	1.0269925893513445
1102	22	0.9906821761093738
1102	23	1.104136269513802
1102	24	1.0695743654028576
1102	25	1.1394877844451727
1102	26	1.1653314115401656
1102	27	1.1189069050983704
1102	28	1.0716004717243166
1102	29	1.1314462439251187
1102	30	1.20357892865389
1102	31	1.1368763593313886
1102	32	1.0872763655179156
1102	33	1.1633188342819096
1102	34	1.0830521207440427
1102	35	1.026781094284928
1102	36	1.087574535255115
1102	37	1.0455261319144142
1102	38	0.9396480483668697
1102	39	1.161522841231644
1102	40	1.0719794719855287
1102	41	1.0850766558332907
1102	42	1.0095608060726704
1102	43	0.9584694349518088
1102	44	0.9264327822000027
1102	45	0.9564247066819433
1102	46	0.859305573032735
1103	1	0.5424812580913247
1103	2	0.46016397852340063
1103	3	0.5768867024612128
1103	4	0.604814074785665
1103	5	0.5643195363749172
1103	6	0.6097996011025018
1103	7	0.5710066896504102
1103	8	0.5708250054604065
1103	9	0.7300040938360226
1103	10	0.6282951879708207
1103	11	0.6769036329457307
1103	12	0.6870120303403908
1103	13	0.6604823382943934
1103	14	0.8978128753951253
1103	15	0.8839883225595173
1103	16	0.9007506935552811
1103	17	0.9008487905221472
1103	18	0.8661850312640027
1103	19	0.7571518080976318
1103	20	0.7142799871889309
1103	21	0.7795080353123893
1103	22	0.8176240208094825
1103	23	0.7770113383021311
1103	24	0.8530706588877166
1103	25	0.8222409061789335
1103	26	0.7280691904148355
1103	27	0.763606143819338
1103	28	0.7148085179294554
1103	29	0.7967703835715554
1103	30	0.7892908830673662
1103	31	0.8782362547344604
1103	32	0.8009578172191567
1103	33	0.869147058698772
1103	34	0.8472181677250248
1103	35	0.8755122475704624
1103	36	0.8026555153938091
1103	37	0.8347759975596816
1103	38	0.8031554027579162
1103	39	0.776835285250506
1103	40	0.6959575544438934
1103	41	0.8437903011187586
1103	42	0.786445848041609
1103	43	0.8617649599404942
1103	44	0.7530531582385933
1103	45	0.7861300058644716
1103	46	0.7549363539913543
1104	1	0.41649399432073864
1104	2	0.439196829554362
1104	3	0.48419075611577966
1104	4	0.5694369002422732
1104	5	0.5597484980410599
1104	6	0.5551811219022582
1104	7	0.5033422080625556
1104	8	0.49998346392943915
1104	9	0.5879380401739098
1104	10	0.699535739108839
1104	11	0.6058746551776792
1104	12	0.6463058957662042
1104	13	0.6702548108299524
1104	14	0.8528338393205672
1104	15	0.8158806454731575
1104	16	0.7635577461896403
1104	17	0.7449380862776636
1104	18	0.7290165355215734
1104	19	0.744244260078105
1104	20	0.8141248491088007
1104	21	0.754108931358239
1104	22	0.7790914861557923
1104	23	0.7635148285158238
1104	24	0.8408625373636269
1104	25	0.676196078098215
1104	26	0.8255516795932252
1104	27	0.7477805186440136
1104	28	0.7775887288140879
1104	29	0.8455681184863977
1104	30	0.887867641351356
1104	31	0.9424207435681637
1104	32	0.8395329653126566
1104	33	0.8203167740347084
1104	34	0.8844181282250361
1104	35	0.8965253121277783
1104	36	0.8854312573708469
1104	37	0.760040751244567
1104	38	0.805760168921779
1104	39	0.7937058045495178
1104	40	0.8117764023525922
1104	41	0.9218382313320959
1104	42	0.9089284315670909
1104	43	0.8385757009174526
1104	44	0.8568297782726333
1104	45	0.788841305627848
1104	46	0.7719520892005605
1105	1	0.04429967130284851
1105	2	-0.010061851464975291
1105	3	0.152826152346627
1105	4	0.14504410129195833
1105	5	0.26432544016218884
1105	6	0.33972111655951664
1105	7	0.18624176319384134
1105	8	0.21874470709950344
1105	9	0.3500965511947882
1105	10	0.2895408590460633
1105	11	0.22775821783626782
1105	12	0.27073373110467003
1105	13	0.3428324132522391
1105	14	0.5336354988325738
1105	15	0.40821542489972273
1105	16	0.3728651902659834
1105	17	0.24365718318413343
1105	18	0.13417094255328976
1105	19	0.279354955081061
1105	20	0.32296154531521637
1105	21	0.2152311355390708
1105	22	0.10385170013196188
1105	23	0.324564241843909
1105	24	0.20676133595086554
1105	25	0.1750240511933967
1105	26	0.2428405752096694
1105	27	0.1580562410838482
1105	28	0.314299873164849
1105	29	0.2778008452446059
1105	30	0.36215008256989023
1105	31	0.3528370403276
1105	32	0.22398045349228113
1105	33	0.33095344936936044
1105	34	0.34370689413947275
1105	35	0.40963543702377336
1105	36	0.404051114626661
1105	37	0.39660851132064323
1105	38	0.37697595379468396
1105	39	0.4021108472474353
1105	40	0.25932598753201574
1105	41	0.32266821702889104
1105	42	0.3068107207877304
1105	43	0.31441938993061314
1105	44	0.28315608664170355
1105	45	0.26003861523750516
1105	46	0.33106600240602546
1106	1	0.09097427539681036
1106	2	0.13150231780168653
1106	3	0.24508125369074563
1106	4	0.17886498291407804
1106	5	0.21161453000982533
1106	6	0.2168913214417998
1106	7	0.24387753501019205
1106	8	0.2790531467276267
1106	9	0.16505762992797912
1106	10	0.3118726215154837
1106	11	0.2673574135580381
1106	12	0.19295441050650908
1106	13	0.24359656963081022
1106	14	0.5494835543802381
1106	15	0.4907096386839953
1106	16	0.3805758741096099
1106	17	0.5207045676305029
1106	18	0.5088886511735069
1106	19	0.3106467790450864
1106	20	0.377600989470281
1106	21	0.39130094296184836
1106	22	0.3463171918854968
1106	23	0.2877308457322769
1106	24	0.3716912301074231
1106	25	0.4430753948919275
1106	26	0.24821076643671347
1106	27	0.4376463384272853
1106	28	0.4283949949458048
1106	29	0.4596836418682315
1106	30	0.44589781660572214
1106	31	0.36373361416676886
1106	32	0.400965852359505
1106	33	0.43315593201454305
1106	34	0.45661424027446174
1106	35	0.31131541499127735
1106	36	0.22344730757672693
1106	37	0.15084328477026077
1106	38	0.25117560719709786
1106	39	0.34674667813420257
1106	40	0.30743945489638236
1106	41	0.45725609347812934
1106	42	0.2826546797637093
1106	43	0.44788653949153456
1106	44	0.41336807271949283
1106	45	0.4338976548139186
1106	46	0.3131449588389046
1107	1	0.361145411338061
1107	2	0.3430327312189583
1107	3	0.3962672352136458
1107	4	0.430807868599949
1107	5	0.400417418425711
1107	6	0.36066096217482624
1107	7	0.36590650616953724
1107	8	0.49341992574490945
1107	9	0.5822244743456224
1107	10	0.43986727548607973
1107	11	0.5587709163323069
1107	12	0.47568601553033574
1107	13	0.4527481199643333
1107	14	0.6805598252997314
1107	15	0.7230111163421213
1107	16	0.6340814683903773
1107	17	0.5870722826529402
1107	18	0.7042575257023174
1107	19	0.7047392965539316
1107	20	0.7215068309524071
1107	21	0.5031757582733292
1107	22	0.559120212302112
1107	23	0.5235558427697597
1107	24	0.6936983011463127
1107	25	0.4661291716876974
1107	26	0.42569414259528315
1107	27	0.6565296422854559
1107	28	0.6095313411957051
1107	29	0.698058952932449
1107	30	0.6842975778044149
1107	31	0.6852453824814901
1107	32	0.7415386755100357
1107	33	0.6171922442142659
1107	34	0.6275701805321319
1107	35	0.5154080375006984
1107	36	0.6067220655878955
1107	37	0.6162301443979885
1107	38	0.5580162448206067
1107	39	0.7140935985422701
1107	40	0.6456731651817833
1107	41	0.6363714786127446
1107	42	0.6570922891705635
1107	43	0.6217193455719681
1107	44	0.7144426786298297
1107	45	0.7020262295210813
1107	46	0.6411250627294791
1108	1	0.3166192477209826
1108	2	0.280691768708472
1108	3	0.2296999489218976
1108	4	0.29299863385194413
1108	5	0.21314073537283917
1108	6	0.16179938748123135
1108	7	0.16496242149745655
1108	8	0.3062878353596119
1108	9	0.21644157077463433
1108	10	0.2730369489868849
1108	11	0.1331306929344649
1108	12	0.016043403536748713
1108	13	0.08162453237902412
1108	14	0.3062215425926098
1108	15	0.2935658379499557
1108	16	0.2871454462401104
1108	17	0.2453895533442818
1108	18	0.28120624774003034
1108	19	0.20521756460822616
1108	20	0.2704708772128157
1108	21	0.2935390686567274
1108	22	0.3113731711794401
1108	23	0.1787297757596562
1108	24	0.31379226182404096
1108	25	0.4159004812668101
1108	26	0.19015384269766855
1108	27	0.20750628558618445
1108	28	0.34600232097064065
1108	29	0.3436371907103961
1108	30	0.15859836175378916
1108	31	0.24253455741637708
1108	32	0.31269849070876177
1108	33	0.30406418335649477
1108	34	0.28186947955381214
1108	35	0.27134760383248135
1108	36	0.30922922444164413
1108	37	0.384752450669815
1108	38	0.28907460536798624
1108	39	0.3075166399977505
1108	40	0.38292228751440405
1108	41	0.4375239153964259
1108	42	0.38702019795324816
1108	43	0.4624339829144972
1108	44	0.4089299278966081
1108	45	0.3760366436599014
1108	46	0.3806816009181162
1109	1	0.09351301031476211
1109	2	0.013953571283579436
1109	3	0.06951620784426638
1109	4	0.013828741660368848
1109	5	0.12358190179853779
1109	6	0.1305750730950095
1109	7	0.08116428053822489
1109	8	0.15429161491585575
1109	9	0.13963703189187981
1109	10	0.1399272678584822
1109	11	0.1085992952955479
1109	12	0.0955293497349885
1109	13	0.13518978095314793
1109	14	0.28397296277225337
1109	15	0.23205939448961033
1109	16	0.2761212661875163
1109	17	0.209307598448504
1109	18	0.18262870422390165
1109	19	0.24297057857664583
1109	20	0.05788663409172301
1109	21	0.10898416263334809
1109	22	0.06033934301263599
1109	23	7.31887529781261E-4
1109	24	0.1416667737262287
1109	25	0.22209462750831221
1109	26	0.20596550809310088
1109	27	0.20193115552163476
1109	28	0.057609656952699595
1109	29	0.1791896377453739
1109	30	0.1993568059882591
1109	31	0.1333345540011166
1109	32	0.24316604003082887
1109	33	0.11417748466226385
1109	34	0.18431053782956042
1109	35	0.20415361127774645
1109	36	0.2148883130221506
1109	37	0.2518281897893573
1109	38	0.28786830648704864
1109	39	0.34091704527546796
1109	40	0.35173653712449493
1109	41	0.34825082538151264
1109	42	0.3554172164298137
1109	43	0.3086521498297188
1109	44	0.29990524124686857
1109	45	0.36564514083092803
1109	46	0.3215672636496478
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2216	18	-2.9777196091519045
2216	19	-2.5879297842774562
2216	20	-2.5408538339336317
2216	21	-2.678599454819505
2216	22	-2.6172765760904895
2216	23	-2.7602588070718923
2216	24	-2.6158387952376287
2216	25	-2.6546641193337237
2216	26	-2.633978320897384
2216	27	-2.8077739466783953
2216	28	-2.7522524717692107
2216	29	-2.926058633468976
2216	30	-2.8157502741483853
2216	31	-2.7745917995641847
2216	32	-2.6696941997251002
2216	33	-2.508767642369868
2216	34	-2.493777788759047
2216	35	-2.4884342721176083
2216	36	-2.662185150823575
2216	37	-2.516214423158523
2216	38	-2.6070231858624737
2216	39	-2.765380946577743
2216	40	-2.679669431211252
2216	41	-2.7994115377182496
2216	42	-2.71723062254663
2216	43	-2.6053188948780246
2216	44	-2.504766712753991
2216	45	-2.656265536640582
2216	46	-2.7261060547888505
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90695.0
3	2857.0
4	8795.0
5	6652.0
6	5343.0
7	4415.0
8	3666.0
9	3483.0
10	3098.0
11	2932.0
12	2920.0
13	3229.0
14	3274.0
15	3428.0
16	3701.0
17	3871.0
18	4378.0
19	5464.0
20	7546.0
21	10434.0
22	13527.0
23	15393.0
24	16578.0
25	16461.0
26	18482.0
27	21149.0
28	23791.0
29	26360.0
30	29502.0
31	35415.0
32	43944.0
33	54656.0
34	70549.0
35	94740.0
36	129495.0
37	188297.0
38	333730.0
39	1103822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75725470241568	18.37115627774619	13.892576285567483	23.979012734270643
2	15.23118583031478	20.426712230343934	39.34438992392296	24.997712015418326
3	18.005972473606388	25.77495123032128	29.778869371813403	26.44020692425893
4	12.45056710387524	17.1168999729566	38.607045282582845	31.825487640585315
5	13.622127188769124	38.006033814536806	34.76631597776615	13.605523018927926
6	30.99377833110934	37.61891201917823	18.117671989907585	13.26963765980484
7	27.45841183540888	32.49096053428872	22.38418391504889	17.666443715253518
8	26.104064779526436	34.54044415894891	20.62860709449056	18.726883967034095
9	26.54229675274578	15.94915217758411	19.5588542063316	37.94969686333851
10	14.338645537053532	29.093793562443505	34.204444238416734	22.36311666208623
11	33.78732090765507	23.753969252571945	24.245221168905562	18.213488670867424
12	23.093724028091877	25.1804168087706	30.647472426318423	21.078386736819102
13	27.473560390584385	22.660831299729477	26.314488972182946	23.551119337503188
14	22.088124857206243	21.675099086451066	27.456425139648154	28.780350916694537
15	23.274707800149745	29.231905214627563	24.207586786635645	23.285800198587044
16	23.113301259233996	28.634742673231596	25.57593482313441	22.6760212444
17	22.110300058276604	28.350118208789173	26.853262165240654	22.686319567693573
18	22.031135014142873	28.108141443624284	27.741180054452336	22.119543487780508
19	23.059778217795554	27.592936279833648	27.816274734444118	21.531010767926677
20	23.31858740398521	27.09667993082879	27.626142678455682	21.95858998673032
21	23.442617590365536	26.92172535268107	27.520609538189888	22.115047518763507
22	23.23581416447854	26.602228741527572	27.509610640742494	22.652346453251393
23	23.095230014411857	26.791031370831757	27.289072401167846	22.824666213588536
24	22.665487336619396	26.913144990958425	27.509363381139796	22.912004291282383
25	22.983866294711223	26.789400925473707	27.49418474706754	22.73254803274753
26	22.75321288880611	27.00320771507212	27.5394548953871	22.70412450073467
27	22.832444964240988	27.104730953539157	27.295045487947505	22.767778594272347
28	22.74673528524321	27.01127993043222	27.426004558659734	22.81598022566483
29	22.963126070189976	27.101587182016214	27.328652677125365	22.606634070668445
30	23.102648591351382	27.03673243685222	27.244138029228832	22.616480942567566
31	23.11753321148064	26.753081085018742	27.35965417676587	22.769731526734745
32	22.904470440984618	26.9977252107574	27.329051409574646	22.76875293868334
33	23.04141018466704	26.877464793396094	27.166038085714376	22.915086936222487
34	23.142871215699003	26.89233158429984	27.40123038028544	22.563566819715717
35	23.1205613874754	26.97099437337698	27.2157363326537	22.69270790649392
36	23.02217636676563	27.039451103560236	27.210815631392787	22.727556898281346
37	23.17986442945464	26.757425086842634	27.260143782928175	22.802566700774545
38	23.06425955001716	26.84804624260229	27.260666426302716	22.827027781077838
39	23.126022567609034	27.12047031794994	27.139891596963324	22.6136155174777
40	23.25117761043707	26.740108548381684	27.360343619253573	22.648370221927667
41	23.073801161308012	26.915990493193537	27.176810614691487	22.833397730806965
42	23.015315907414607	27.018166803951992	27.089936177852213	22.876581110781185
43	23.011762932995623	26.78473621478893	27.236905131053224	22.966595721162225
44	23.05527678967694	26.986082682511608	27.094635771679155	22.864004756132296
45	23.19093961801561	27.051811285650352	26.90570947972605	22.851539616607997
46	23.186703638722626	26.810729624039233	26.899571851669084	23.102994885569057
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	792.0
1	769.0
2	746.0
3	875.5
4	1005.0
5	1084.0
6	1163.0
7	1163.0
8	1141.0
9	1119.0
10	1324.5
11	1530.0
12	2174.5
13	2819.0
14	5207.5
15	7596.0
16	10401.5
17	13207.0
18	14265.5
19	15324.0
20	15324.0
21	17411.0
22	19498.0
23	23981.0
24	28464.0
25	35203.5
26	41943.0
27	48987.0
28	56031.0
29	63628.5
30	71226.0
31	71226.0
32	79108.0
33	86990.0
34	96596.5
35	106203.0
36	114275.0
37	122347.0
38	129714.5
39	137082.0
40	145266.5
41	153451.0
42	158817.0
43	164183.0
44	164183.0
45	169019.0
46	173855.0
47	177090.0
48	180325.0
49	180719.0
50	181113.0
51	177186.0
52	173259.0
53	166068.5
54	158878.0
55	149226.0
56	139574.0
57	139574.0
58	127966.0
59	116358.0
60	103138.0
61	89918.0
62	77313.5
63	64709.0
64	54014.0
65	43319.0
66	34968.5
67	26618.0
68	21206.0
69	15794.0
70	15794.0
71	12425.0
72	9056.0
73	7100.0
74	5144.0
75	3901.0
76	2658.0
77	2027.5
78	1397.0
79	1052.5
80	708.0
81	708.0
82	532.0
83	356.0
84	252.5
85	149.0
86	110.0
87	71.0
88	51.5
89	32.0
90	23.5
91	15.0
92	13.5
93	12.0
94	12.0
95	13.5
96	15.0
97	10.5
98	6.0
99	10.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19473757404580658
2	0.05310272210430815
3	0.0687893407150118
4	0.05939392534659563
5	0.04205172693529001
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.2416848504514765E-4
16	0.0
17	3.3111596012039377E-4
18	5.794529302106891E-4
19	3.3111596012039377E-4
20	0.0015728008105718705
21	0.0032697701061888884
22	0.0
23	2.483369700902953E-4
24	4.552844451655414E-4
25	4.966739401805906E-4
26	9.105688903310828E-4
27	0.024709528523984385
28	4.552844451655414E-4
29	0.0012002953554364273
30	0.0013658533354966244
31	0.001655579800601969
32	9.933478803611812E-4
33	0.0019866957607223624
34	0.0029386541460684947
35	0.0024005907108728547
36	0.031166289746332062
37	0.008609014963130239
38	0.012044343049379323
39	0.049460446542983816
40	0.015521060630643459
41	0.05715889261578297
42	0.05765556655596356
43	0.02495786549407468
44	0.02748262468999268
45	0.026199550344526156
46	0.02292978023833727
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2416072.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.86754175218225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97573073325493	53.4778051396721
2	12.040512861124723	16.102389929178894
3	3.640823414353104	7.303587350147367
4	1.5402847790357999	4.119802270897086
5	0.8296664961936318	2.7738879537307213
6	0.4966853883680607	1.9927278566640092
7	0.3103261637242343	1.452552339673735
8	0.22147365891054682	1.1847519307367647
9	0.16550182133134406	0.9960029953142271
>10	0.749985995526038	8.469082793607068
>50	0.023028996746744093	1.0273698955558728
>100	0.005731372392099862	0.5946820837857414
>500	6.207975967358298E-5	0.025487882460703926
>1k	1.2415951934706445E-4	0.23686295980563143
>5k	6.207975967353222E-5	0.24300661877009574
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5854	0.24229410381809816	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2880	0.11920174564334177	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2826	0.11696671291252911	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2416848504514765E-4
2	0.0	0.0	0.0	0.0	1.2416848504514765E-4
3	0.0	0.0	0.0	0.0	1.2416848504514765E-4
4	0.0	0.0	0.0	0.0	1.2416848504514765E-4
5	0.0	0.0	0.0	0.0	1.2416848504514765E-4
6	0.0	0.0	0.0	0.0	1.2416848504514765E-4
7	0.0	0.0	0.0	0.0	1.2416848504514765E-4
8	0.0	0.0	0.0	0.0	1.6555798006019688E-4
9	0.0	0.0	0.0	0.0	1.6555798006019688E-4
10	0.0	0.0	0.0	0.0	1.6555798006019688E-4
11	0.0	0.0	0.0	4.138949501504922E-5	1.6555798006019688E-4
12	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	2.8972646510534456E-4
13	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	3.3111596012039377E-4
14	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	3.3111596012039377E-4
15	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	3.72505455135443E-4
16	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	3.72505455135443E-4
17	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	3.72505455135443E-4
18	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	3.72505455135443E-4
19	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	3.72505455135443E-4
20	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	4.138949501504922E-4
21	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	4.138949501504922E-4
22	8.277899003009844E-5	0.0	0.0	8.277899003009844E-5	4.138949501504922E-4
23	8.277899003009844E-5	0.0	0.0	1.2416848504514765E-4	4.138949501504922E-4
24	8.277899003009844E-5	0.0	0.0	1.6555798006019688E-4	4.138949501504922E-4
25	8.277899003009844E-5	0.0	0.0	1.6555798006019688E-4	4.138949501504922E-4
26	8.277899003009844E-5	0.0	0.0	2.069474750752461E-4	4.5528444516554145E-4
27	8.277899003009844E-5	0.0	0.0	3.3111596012039377E-4	4.5528444516554145E-4
28	8.277899003009844E-5	0.0	0.0	6.208424252257383E-4	4.5528444516554145E-4
29	8.277899003009844E-5	0.0	0.0	0.001117516365406329	4.5528444516554145E-4
30	8.277899003009844E-5	0.0	0.0	0.0016555798006019687	4.5528444516554145E-4
31	8.277899003009844E-5	0.0	0.0	0.0038492230363995775	4.966739401805906E-4
32	8.277899003009844E-5	0.0	0.0	0.005463413341986497	4.966739401805906E-4
33	8.277899003009844E-5	0.0	0.0	0.007201772132618564	4.966739401805906E-4
34	8.277899003009844E-5	0.0	0.0	0.01009903678367201	4.966739401805906E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1400	0.0	27.056713	1
GTATCAA	3730	0.0	24.662958	1
TATACCG	75	1.13879614E-4	18.666765	5
TATCAAC	5215	0.0	17.386372	2
ATCAACG	5325	0.0	16.794035	3
TCAACGC	5375	0.0	16.598871	4
CAACGCA	5485	0.0	16.262619	5
AACGCAG	5695	0.0	15.656784	6
CGTGCGC	180	1.8655555E-8	14.438839	10
ACGCAGA	6635	0.0	13.40851	7
GAGTACT	3075	0.0	13.393729	12
AACCGCG	90	0.0081792055	13.338649	38
CCGATAA	90	0.008221844	13.328159	9
ATTATAC	590	0.0	13.224513	3
GTACTAA	305	0.0	13.1423025	1
CGCAGAG	6785	0.0	12.964753	8
GTACATG	6895	0.0	12.44087	1
GCACCGT	225	2.568595E-8	12.439616	6
AGTACTT	3310	0.0	12.322012	13
CGAATCG	130	5.1990943E-4	12.312345	37
>>END_MODULE