FastQCFastQC Report
Mon 6 Feb 2017
SRR2077354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416072
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21800.041.668481
GTATCAA51300.039.0636981
TATCAAC69900.028.161342
TCAACGC71000.027.625694
ATCAACG71150.027.5187423
CAACGCA72000.027.1899095
AACGCAG75250.025.969016
ACGCAGA87550.022.150237
CGCAGAG89050.021.690198
GAGTACT60200.020.51227212
AGAGTAC89750.020.48424511
CAGAGTA95600.019.5911610
AGTACTT64950.019.11835713
GCAGAGT99900.019.0241099
GTACCGA3300.018.899116
TATACCG1852.528759E-718.7292375
GTACATG64600.018.6771071
GTACTTT66350.018.50164814
CGAATCG1702.4476703E-618.25441737
TACATGG66800.018.0025962
Produced by FastQC (version 0.11.5)